PAH Antibody - #DF6455

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产品: PAH 抗体
货号: DF6455
描述: Rabbit polyclonal antibody to PAH
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52kDa, 70 kDa; 52kD(Calculated).
蛋白号: P00439
RRID: AB_2838418

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)
克隆:
Polyclonal
特异性:
PAH Antibody detects endogenous levels of total PAH.
RRID:
AB_2838418
引用格式: Affinity Biosciences Cat# DF6455, RRID:AB_2838418.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

PAH; PH; PH4H_HUMAN; Phe 4 monooxygenase; Phe-4-monooxygenase; Phenylalanine 4 hydroxylase; Phenylalanine hydroxylase; Phenylalanine-4-hydroxylase; PKU; PKU1;

抗原和靶标

免疫原:
Uniprot:

P00439(PH4H_HUMAN) >>Visit HPA database.

基因/基因ID:
PAH(5053)
描述:
PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
序列:
MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
88
Sheep
88
Xenopus
88
Chicken
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00439 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
T3 Phosphorylation
S16 Phosphorylation P17612 (PRKACA)
S87 Phosphorylation
T92 Phosphorylation
T117 Phosphorylation
S436 Phosphorylation

研究背景

功能:

Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.

翻译修饰:

Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.

亚基结构:

Homodimer and homotetramer.

蛋白家族:

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

研究领域

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine, tyrosine and tryptophan biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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