| 产品: | B2M 抗体 |
| 货号: | DF6458 |
| 描述: | Rabbit polyclonal antibody to B2M |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit |
| 分子量: | 14kDa; 14kD(Calculated). |
| 蛋白号: | P61769 |
| RRID: | AB_2838420 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6458, RRID:AB_2838420.
展开/折叠
B2M; B2MG_HUMAN; Beta 2 microglobin; Beta 2 microglobulin; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; CDABP0092; Hdcma22p;
抗原和靶标
- P61769 B2MG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61769 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| C45 | S-Nitrosylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K78 | Methylation | Uniprot | |
| T106 | O-Glycosylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot |
研究背景
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation.
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Secreted. Cell surface.
Note: Detected in serum and urine (PubMed:1336137, PubMed:7554280).
Note: (Microbial infection) In the presence of M.tuberculosis EsxA-EsxB complex decreased amounts of B2M are found on the cell surface (PubMed:25356553).
Heterodimer of an alpha chain and a beta chain. Beta-2-microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. B2M alone (not in complex with HLA-I) interacts with M.tuberculosis EsxA (ESAT-6) and an EsxA-EsxB (CFP-10) complex; the tripartite complex can be detected in the host endoplasmic reticulum. The B2M-EsxA complex can be detected in patients with pleural tuberculosis and is stable from pH 4.0 to 8.0 and in the presence of 2M NaCl. Forms a heterotrimer with HLA-E and a self- or a foreign peptide. Forms a heterotrimer with HLA-G and a self-peptide. Forms a heterotrimer with HLA-F and a self-peptide.
Belongs to the beta-2-microglobulin family.
研究领域
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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