IL6R Antibody - #DF6466

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产品: IL6R 抗体
货号: DF6466
描述: Rabbit polyclonal antibody to IL6R
应用: WB IHC
反应: Human, Mouse
分子量: 52kD; 52kD(Calculated).
蛋白号: P08887
RRID: AB_2838428

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
IL6R Antibody detects endogenous levels of total IL6R.
RRID:
AB_2838428
引用格式: Affinity Biosciences Cat# DF6466, RRID:AB_2838428.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD 126; CD126; CD126 antigen; gp80; IL 6R 1; IL 6R alpha; IL 6R; IL-6 receptor alpha chain; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL6Q; Il6r; IL6RA; IL6RA_HUMAN; IL6RQ; Interleukin 6 receptor; interleukin 6 receptor, alpha; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC30256;

抗原和靶标

免疫原:
Uniprot:

P08887(IL6RA_HUMAN) >>Visit HPA database.

基因/基因ID:
IL6R(3570)
表达:
P08887 IL6RA_HUMAN:

Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

描述:
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R has been found in human serum and urine.
序列:
MLAVGCALLAALLAAPGAALAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLPTFLVAGGSLAFGTLLCIAIVLRFKKTWKLRALKEGKTSMHPPYSLGQLVPERPRPTPVLVPLISPPVSPSSLGSDNTSSHNRPDARDPRSPYDISNTDYFFPR

翻译修饰 - P08887 作为底物

Site PTM Type Enzyme
N55 N-Glycosylation
S91 Phosphorylation
N93 N-Glycosylation
T139 Phosphorylation
S141 Phosphorylation
T144 Phosphorylation
N221 N-Glycosylation
S243 Phosphorylation
S455 Phosphorylation
Y457 Phosphorylation
Y464 Phosphorylation

研究背景

功能:

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.

Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.

翻译修饰:

A short soluble form may also be released from the membrane by proteolysis.

细胞定位:

Basolateral cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

亚基结构:

Hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts (via N-terminal ectodomain) with SORL1; this interaction may affect IL6-binding to IL6R, hence decrease IL6 cis-signaling. The soluble form of IL6R also interacts with SORL1; this interaction leads to soluble IL6R internalization. May form a trimeric complex with the soluble SORL1 ectodomain and circulating IL6 receptor; this interaction might stabilize circulating IL6, hence promote IL6 trans signaling.

蛋白家族:

The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

Belongs to the type I cytokine receptor family. Type 3 subfamily.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

文献引用

1). Macrophage Membrane-Derived Biomimetic Nanoparticles for Treatment of Cytokine Release Syndrome. Journal of Biomedical Nanotechnology (PubMed: 35854441) [IF=2.9]
2). Antinociceptive effects of IL-6R vs. glucocorticoid receptors during rat hind paw inflammatory pain. NEUROSCIENCE LETTERS (PubMed: 32898615) [IF=2.5]

Application: WB    Species: rat    Sample: spinal cord

Fig. 4. |IL-6-induced signaling pathway test was assessed in the spinal cord by Western blotting, and the IL-6 and sIL-6R concentration levels in the serum and CSF were tested by ELISA. A–H: Levels of IL-6, IL-6Rα, gp130, JAK2, pJAK2, STAT3, and pSTAT3 proteins in the spinal cord following three consecutive days of i.th. injection of anti-IL-6R (20 μg/d), sgp130 (20 μg/d), and Dexamethasone (20 μg/d).
3). Therapeutic Effect of Macrophage-Derived Biomimetic Nanoparticles for Cytokine Release Syndrome.

Application: WB    Species: Mouse    Sample:

Figure 2. Characterization of biomimetic nanoparticles. (A) The particle size and zeta potential of PLGA nanoparticles and PNP@MP were detected by DLS; (B) Particle size distribution range of PLGA and PNP@MP; (C) Transmission electron microscope image of PNP@MP negatively stained with phosphotungstic acid; (D) Stability of PNP@MP in PBS or PBS containing 10% FBS within 72 h;(E) Characteristic protein bands of macrophage cell lysates, membrane-derived biomimetic nanoparticles resolved by Western blotting.

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