HP Antibody - #DF6467

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产品: HP 抗体
货号: DF6467
描述: Rabbit polyclonal antibody to HP
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Rabbit, Dog
分子量: 42kDa; 45kD(Calculated).
蛋白号: P00738
RRID: AB_2838429

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Rabbit(80%), Dog(90%)
克隆:
Polyclonal
特异性:
HP Antibody detects endogenous levels of total HP.
RRID:
AB_2838429
引用格式: Affinity Biosciences Cat# DF6467, RRID:AB_2838429.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Binding peptide; BP; Haptoglobin alpha chain; Haptoglobin alpha(1S) beta; Haptoglobin alpha(2FS) beta; Haptoglobin beta chain; Haptoglobin, alpha polypeptide; Haptoglobin, beta polypeptide; HP; HP2 ALPHA2; HP2ALPHA2; HPA1S; HPT; HPT_HUMAN; MGC111141; Zonulin;

抗原和靶标

免疫原:
Uniprot:

P00738(HPT_HUMAN) >>Visit HPA database.

基因/基因ID:
HP(3240)
表达:
P00738 HPT_HUMAN:

Expressed by the liver and secreted in plasma.

描述:
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
序列:
MSALGAVIALLLWGQLFAVDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
90
Rabbit
80
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00738 作为底物

Site PTM Type Enzyme
Y55 Phosphorylation
Y56 Phosphorylation
Y66 Phosphorylation
Y114 Phosphorylation
Y115 Phosphorylation
Y125 Phosphorylation
K170 Acetylation
N184 N-Glycosylation
N207 N-Glycosylation
N211 N-Glycosylation
N241 N-Glycosylation
K251 Ubiquitination
C266 S-Nitrosylation
Y272 Phosphorylation
Y280 Phosphorylation
C309 S-Nitrosylation
K321 Acetylation
S326 Phosphorylation
C340 S-Nitrosylation
C381 S-Nitrosylation
Y386 Phosphorylation

研究背景

功能:

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed by the liver and secreted in plasma.

亚基结构:

Tetramer of two alpha and two beta chains; disulfide-linked. The hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163.

蛋白家族:

Belongs to the peptidase S1 family.

文献引用

1). Huangqin Qingre Chubi Capsule inhibits rheumatoid arthritis by regulating intestinal flora and improving intestinal barrier. Frontiers in pharmacology, 2024 (PubMed: 38989143) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 4 HQC treatment restored intestinal barrier damage in the large intestine in CIA mice (A), The mRNA expression of ZO-1, Occludin, and Claudin-1 in large intestine tissue; (B), The mRNA expression of Zonulin in large intestine tissue; (C) The protein expression of Occludin, and Claudin-1 in large intestine tissue; (D), Immunofluorescence detection of ZO-1 expression in large intestine tissue (scale bar = 50 μm); (E), Immunofluorescence detection of Zonulin expression in large intestine tissue (scale bar = 50 μm); *p < 0.05 vs. Normal, #p < 0.05 vs. CIA mice; for (A–C), n = 5; for (D and E), n = 3.
2). TMT-based quantitative proteomic analysis of hepatic tissue reveals the effects of dietary cyanidin-3-diglucoside-5-glucoside-rich extract on alleviating D-galactose-induced aging in mice. Journal of Proteomics, 2021 (PubMed: 33161165) [IF=3.3]

Application: WB    Species: mice    Sample: Liver tissues

Fig. 5. Western blot analysis of three differentially expressed proteins. Data are presented as means ± SD (n = 3 per group). Significance was determined by ANOVA followed by Tukey’s post hoc correction. *p < 0.05 vs NC, #p < 0.05 vs DG; Two-way ANOVA for groups (A) F = 13.8, df = 8; (B) F = 195.3, df = 8; (C) F = 23.1, df = 8.

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