alpha 2 Macroglobulin/A2M Antibody - #DF6469

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产品: alpha 2 Macroglobulin/A2M 抗体
货号: DF6469
描述: Rabbit polyclonal antibody to alpha 2 Macroglobulin/A2M
应用: WB IHC
反应: Human, Mouse
分子量: 163kDa; 163kD(Calculated).
蛋白号: P01023
RRID: AB_2838431

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
alpha 2 Macroglobulin/A2M Antibody detects endogenous levels of total alpha 2 Macroglobulin/A2M.
RRID:
AB_2838431
引用格式: Affinity Biosciences Cat# DF6469, RRID:AB_2838431.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A2m; A2MG_HUMAN; Alpha 2 M; Alpha 2M; Alpha-2-M; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; DKFZp779B086; FWP007; S863 7;

抗原和靶标

免疫原:
Uniprot:

P01023(A2MG_HUMAN) >>Visit HPA database.

基因/基因ID:
A2M(2)
表达:
P01023 A2MG_HUMAN:

Secreted in plasma.

描述:
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
序列:
MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

翻译修饰 - P01023 作为底物

Site PTM Type Enzyme
N55 N-Glycosylation
N70 N-Glycosylation
N247 N-Glycosylation
T346 Phosphorylation
N396 N-Glycosylation
N410 N-Glycosylation
Y497 Phosphorylation
T679 Phosphorylation
Y695 Phosphorylation
Y708 Phosphorylation
S710 Phosphorylation
Y817 Phosphorylation
K820 Acetylation
T861 Phosphorylation
N869 N-Glycosylation
T898 Phosphorylation
N991 N-Glycosylation
Y1007 Phosphorylation
Y1012 Phosphorylation
Y1021 Phosphorylation
Y1025 Phosphorylation
K1147 Acetylation
K1162 Acetylation
K1164 Acetylation
K1168 Acetylation
S1387 Phosphorylation
T1395 Phosphorylation
N1424 N-Glycosylation

研究背景

功能:

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Secreted in plasma.

亚基结构:

Homotetramer; disulfide-linked.

蛋白家族:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

研究领域

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

文献引用

1). Comparison of the sensitivity of Western blotting between PVDF and NC membranes. Scientific Reports, 2021 (PubMed: 34103620) [IF=4.6]

Application: WB    Species: Human    Sample:

Figure 5. Comparison of the re-probed ability of PVDF membrane and NC membrane. Te pooled sera proteins (3.0, 1.5, 0.7, 0.3 and 0.1 μg) were separated by 8% SDS-PAGE, and transferred to PVDF membranes (up) and NC membrane (down), respectively. (a) Staining with AAL and then re-probed with PHA-E; (b) staining with ApoA1 and then re-probing with IgG; (c) Staining with PHA-E and then re-probing with A2M; (d) staining with A2M and then re-probing with PHA-E. Band intensities were statistically analyzed (n=3 individual experiments) and compared using Image Lab sofware (Bio-Rad Laboratories) and GraphPad Prism version 6. Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed *Signifcantly diferent p<0.05, **p<0.01. N.S., not signifcant. All values are means±S.E. (error bars).

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