产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6474, RRID:AB_2838436.
展开/折叠
Christmas Disease; Christmas factor; Coagulant factor IX; Coagulation factor 9; Coagulation factor IX; Coagulation factor IXa heavy chain; F9; FA9_HUMAN; Factor 9; Factor IX Deficiency; FactorIX; FIX; Haemophilia B; HEMB; MGC129641; MGC129642; P19; Plasma Thromboplastic Component; Plasma thromboplastin component; PTC;
抗原和靶标
Detected in blood plasma (at protein level) (PubMed:3857619, PubMed:8295821, PubMed:2592373, PubMed:9169594, PubMed:19846852). Synthesized primarily in the liver and secreted in plasma.
- P00740 FA9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00740 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T85 | O-Glycosylation | Uniprot | |
| S99 | O-Glycosylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| T205 | O-Glycosylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| T215 | O-Glycosylation | Uniprot | |
| T225 | O-Glycosylation | Uniprot |
研究背景
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro. Xylosylation at this site is minor.
Secreted.
Detected in blood plasma (at protein level). Synthesized primarily in the liver and secreted in plasma.
Heterodimer of a light chain and a heavy chain; disulfide-linked. Interacts with SERPINC1.
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues in the Gla domain. Calcium can also bind, with stronger affinity, to another site beyond the Gla domain (PubMed:6425296). Under physiological ion concentrations, Ca(2+) is displaced by Mg(2+) from some of the gammaglutamate residues in the N-terminal Gla domain. This leads to a subtle conformation change that may affect the interaction with its binding protein (By similarity).
Belongs to the peptidase S1 family.
研究领域
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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