产品: | ITGAM 抗体 |
货号: | DF6476 |
描述: | Rabbit polyclonal antibody to ITGAM |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Bovine, Horse, Sheep, Rabbit, Dog |
分子量: | 127kDa; 127kD(Calculated). |
蛋白号: | P11215 |
RRID: | AB_2838438 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6476, RRID:AB_2838438.
展开/折叠
antigen CD11b (p170); Antigen CD11b p170; CD11 antigen like family member B; CD11 antigen-like family member B; CD11b; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; Complement Component Receptor 3 Alpha; Complement receptor type 3, alpha subunit; CR 3 alpha chain (CR3A); CR 3 alpha chain; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; Integrin Alpha M; Integrin alpha M chain; Integrin alpha-M; Integrin beta 2 alpha subunit; Integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM_HUMAN; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; MAC 1; Mac-1a; MAC1; Mac1, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MGC117044; Mo1, alpha subunit; MO1A; Neutrophil adherence receptor alpha M subunit; Neutrophil adherence receptor; SLEB6;
抗原和靶标
Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).
- P11215 ITAM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRVLLLTALTLCHGFNLDTENAMTFQENARGFGQSVVQLQGSRVVVGAPQEIVAANQRGSLYQCDYSTGSCEPIRLQVPVEAVNMSLGLSLAATTSPPQLLACGPTVHQTCSENTYVKGLCFLFGSNLRQQPQKFPEALRGCPQEDSDIAFLIDGSGSIIPHDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKIFAIEGTQTGSSSSFEHEMSQEGFSAAITSNGPLLSTVGSYDWAGGVFLYTSKEKSTFINMTRVDSDMNDAYLGYAAAIILRNRVQSLVLGAPRYQHIGLVAMFRQNTGMWESNANVKGTQIGAYFGASLCSVDVDSNGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGRARWQCDAVLYGEQGQPWGRFGAALTVLGDVNGDKLTDVAIGAPGEEDNRGAVYLFHGTSGSGISPSHSQRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAGEVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVGTPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYRKVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLELPVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSDFLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEPFEVPNPLPLIVGSSVGGLLLLALITAALYKLGFFKRQYKDMMSEGGPPGAEPQ
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11215 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S574 | Phosphorylation | Uniprot | |
S577 | Phosphorylation | Uniprot | |
Y676 | Phosphorylation | Uniprot | |
T805 | Phosphorylation | Uniprot | |
N900 | N-Glycosylation | Uniprot | |
T930 | Phosphorylation | Uniprot | |
S931 | Phosphorylation | Uniprot | |
N940 | N-Glycosylation | Uniprot | |
N946 | N-Glycosylation | Uniprot | |
S1142 | Phosphorylation | Uniprot |
研究背景
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Cell membrane>Single-pass type I membrane protein. Membrane raft>Single-pass type I membrane protein.
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD. Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Belongs to the integrin alpha chain family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: WB Species: Rat Sample: hippocampus
Application: WB Species: rat Sample:
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