(2)
(2)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6478, RRID:AB_2838440.
展开/折叠
BXR; Nr1i2; NR1I2_HUMAN; Nuclear receptor subfamily 1 group I member 2; ONR 1; ONR1; Orphan nuclear receptor PAR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; OTTHUMP00000215173; OTTHUMP00000215174; OTTHUMP00000215175; PAR 1; PAR 2; PAR; PAR q; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; Pregnane X receptor; PRR; PXR; SAR; Steroid and xenobiotic receptor; SXR;
抗原和靶标
- O75469 NR1I2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75469 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K109 | Acetylation | Uniprot | |
| S114 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T133 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T135 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S167 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S200 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S208 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T408 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T422 | Phosphorylation | Uniprot |
研究背景
Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
Nucleus.
Expressed in liver, colon and small intestine.
Heterodimer with RXR. Interacts with NCOA1. Interacts (via domain NR LBD) with CRY1 and CRY2 in a ligand-dependent manner (By similarity).
Belongs to the nuclear hormone receptor family. NR1 subfamily.
文献引用
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