产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6495, RRID:AB_2838457.
展开/折叠
DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3;
抗原和靶标
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
- P00374 DYR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00374 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| R29 | Methylation | Uniprot | |
| R33 | Methylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K81 | Sumoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K156 | Sumoylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| Y157 | Phosphorylation | Uniprot | |
| K158 | Sumoylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Sumoylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K179 | Sumoylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot |
研究背景
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Mitochondrion. Cytoplasm.
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Homodimer.
Belongs to the dihydrofolate reductase family.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.
· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.
· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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