NPPA Antibody - #DF6497

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产品: NPPA 抗体
货号: DF6497
描述: Rabbit polyclonal antibody to NPPA
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 17kDa; 16kD(Calculated).
蛋白号: P01160
RRID: AB_2838459

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
NPPA Antibody detects endogenous levels of total NPPA.
RRID:
AB_2838459
引用格式: Affinity Biosciences Cat# DF6497, RRID:AB_2838459.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ANF; ANF_HUMAN; ANP; ATFB6; Atrial natriuretic factor; Atrial natriuretic peptide; Atriopeptin; Cardiodilatin; Cardiodilatin-related peptide; Cardionatrin; CDD ANF; CDD; CDD-ANF; CDP; Natriuretic peptide A; Natriuretic peptide precursor A; NPPA; PND; Prepronatriodilatin; ANP;

抗原和靶标

免疫原:
Uniprot:

P01160(ANF_HUMAN) >>Visit HPA database.

基因/基因ID:
NPPA(4878)
描述:
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]
序列:
MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
73
Chicken
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01160 作为底物

Site PTM Type Enzyme
K41 Acetylation
Y151 Phosphorylation

研究背景

功能:

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

翻译修饰:

Cleaved by CORIN upon secretion to produce the functional hormone.

Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the natriuretic peptide family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

文献引用

1). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]
2). 16α-OHE1 alleviates hypoxia-induced inflammation and myocardial damage via the activation of β2-Adrenergic receptor. Molecular and cellular endocrinology (PubMed: 38518841) [IF=4.1]
3). Canagliflozin mediates mitophagy through the AMPK/PINK1/PARKIN pathway to alleviate isoprenaline-induced cardiac remodelling. Journal of cardiovascular pharmacology, 2024 (PubMed: 39150485) [IF=3.0]
4). Metformin attenuates angiotensin II‑induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway. Experimental and Therapeutic Medicine, 2021 (PubMed: 34539827) [IF=2.7]

Application: WB    Species: Rat    Sample: H9c2 cells

Figure 1 Effect of metformin on cardiomyocyte hypertrophy. (A) Metformin reversed the changes in the surface area of cardiomyocytes induced by Ang II treatment for 48 h. Metformin reversed the changes in (B) ANP and (C) BNP mRNA expression in cardiomyocytes after treatment with Ang II for 48 h. (D) Western blot analysis of ANP and BNP. (E) Densitometric analysis of ANP and BNP expression in cardiomyocytes. n=3 for each group. *P<0.05. #P<0.05. ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; Ang, angiotensin; Met, metformin.
5). A Novel Mechanism of 16α-OHE1, One of Estrogen Metabolites, Alleviating Inflammatory Infiltration in Hypoxia-Induced Myocardial Injury via β2-Adrenergic Receptor. Research Square, 2023

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