产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6498, RRID:AB_2838460.
展开/折叠
ABC transporter, MHC 2; ABC18; ABCB3; Antigen peptide transporter 2; APT2; ATP binding cassette, sub family B (MDR/TAP), member 3; ATP-binding cassette sub-family B member 3; D6S217E; Peptide supply factor 2; Peptide transporter involved in antigen processing 2; Peptide transporter PSF2; Peptide transporter TAP2; PSF 2; PSF-2; PSF2; Really interesting new gene 11 protein; RING 11; RING11; TAP 2; Tap2; TAP2_HUMAN; Transporter 2 ATP binding cassette sub family B; Transporter 2, ABC (ATP binding cassette; Transporter 2, ATP binding cassette, sub family B (MDR/TAP);
抗原和靶标
- Q03519 TAP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLCLATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWRLLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVLKGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSVRNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLILDEATSALDVQCEQALQDWNSRGDRTVLVIAHRLQTVQRAHQILVLQEGKLQKLAQL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q03519 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K54 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T492 | Phosphorylation | Uniprot | |
| Y597 | Phosphorylation | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K682 | Ubiquitination | Uniprot |
研究背景
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: The transmembrane segments seem to form a pore in the membrane.
Heterodimer of TAP1 and TAP2. Interacts with HLA-A*02-B2M complex; this interaction is obligatory for the loading of peptide epitopes delivered to the ER by TAP1-TAP2 transporter. Similarly to classical MHC class I assembly, HLA-E-B2M complex binds TAP1-TAP2 heterodimer and CALR before the peptide loading. Interacts with HLA-F.
(Microbial infection) Interacts with Epstein-Barr virus BLNF2a.
(Microbial infection) Interacts with herpes simplex virus ICP47.
The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Membrane transport > ABC transporters.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
限制条款
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