Insulin degrading enzyme/IDE Antibody - #DF6515
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| 产品: | Insulin degrading enzyme/IDE 抗体 |
| 货号: | DF6515 |
| 描述: | Rabbit polyclonal antibody to Insulin degrading enzyme/IDE |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 118kDa; 118kD(Calculated). |
| 蛋白号: | P14735 |
| RRID: | AB_2838477 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6515, RRID:AB_2838477.
展开/折叠
Abeta-degrading protease; FLJ35968; Ide; IDE_HUMAN; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin; OTTHUMP00000020097;
抗原和靶标
- P14735 IDE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P14735 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| T55 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K308 | Acetylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K511 | Ubiquitination | Uniprot | |
| K512 | Acetylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K521 | Acetylation | Uniprot | |
| K527 | Acetylation | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| Y625 | Phosphorylation | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| T691 | Phosphorylation | Uniprot | |
| T696 | Phosphorylation | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| R774 | Methylation | Uniprot | |
| K826 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K884 | Ubiquitination | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| S901 | Phosphorylation | Uniprot | |
| K929 | Ubiquitination | Uniprot | |
| K933 | Ubiquitination | Uniprot | |
| K938 | Ubiquitination | Uniprot | |
| S955 | Phosphorylation | Uniprot | |
| K1018 | Ubiquitination | Uniprot |
研究背景
Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.
(Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
The N-terminus is blocked.
Cytoplasm>Cytosol. Cell membrane. Secreted.
Note: Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Detected in brain and in cerebrospinal fluid (at protein level).
Homodimer (Probable). Can also form homotetramers (By similarity).
(Microbial infection) Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.
The SlyX motif may be involved in the non-conventional secretion of the protein.
Belongs to the peptidase M16 family.
研究领域
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
文献引用
Application: WB Species: rat Sample: BV-2 cells
Application: WB Species: rat Sample: BV-2 cell
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