PPP1R2 Antibody - #AF0799

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产品: PPP1R2 抗体
货号: AF0799
描述: Rabbit polyclonal antibody to PPP1R2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25kDa; 23kD(Calculated).
蛋白号: P41236
RRID: AB_2833792

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PPP1R2 Antibody detects endogenous levels of total PPP1R2.
RRID:
AB_2833792
引用格式: Affinity Biosciences Cat# AF0799, RRID:AB_2833792.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IPP 2; IPP-2; IPP2; IPP2_HUMAN; MGC87148; Phosphatase inhibitor 2; Phosphoprotein phosphatase; Ppp1r2; PPP1R2 protein; Protein phosphatase 1 regulatory (inhibitor) subunit 2; Protein phosphatase 1 regulatory subunit 2; Protein phosphatase inhibitor 2;

抗原和靶标

免疫原:
Uniprot:

P41236(IPP2_HUMAN) >>Visit HPA database.

基因/基因ID:
PPP1R2(5504)
描述:
PPP1R2 a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32.
序列:
MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKSQKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILARKLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQLISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P41236 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
S7 Phosphorylation
T19 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
R33 Methylation
S44 Phosphorylation Q13315 (ATM)
T55 Phosphorylation
Y56 Phosphorylation
Y63 Phosphorylation
S72 Phosphorylation P27448 (MARK3)
T73 Phosphorylation P49841 (GSK3B) , Q00535 (CDK5)
Y75 Phosphorylation
S77 Phosphorylation
S87 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2) , O94921 (CDK14)
T89 Phosphorylation
T92 Phosphorylation
K103 Ubiquitination
K113 Ubiquitination
Y114 Phosphorylation
S121 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S122 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S127 Phosphorylation
S130 Phosphorylation
K145 Ubiquitination
Y148 Phosphorylation
K155 Ubiquitination
T193 Phosphorylation

研究背景

功能:

Inhibitor of protein-phosphatase 1.

翻译修饰:

Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.

亚基结构:

Heterodimer with PP1.

蛋白家族:

Belongs to the protein phosphatase inhibitor 2 family.

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