| 产品: | VTN 抗体 |
| 货号: | DF6542 |
| 描述: | Rabbit polyclonal antibody to VTN |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 分子量: | 54kDa; 54kD(Calculated). |
| 蛋白号: | P04004 |
| RRID: | AB_2838504 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6542, RRID:AB_2838504.
展开/折叠
Complement S Protein; Epibolin; S Protein; S-protein; Serum Spreading Factor; Serum-spreading factor; Somatomedin B; Somatomedin-B; V75; Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN; VTNC_HUMAN;
抗原和靶标
Expressed in the retina pigment epithelium (at protein level) (PubMed:25136834). Plasma.
- P04004 VTNC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPLRPLLILALLAWVALADQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
翻译修饰 - P04004 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y75 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| N86 | N-Glycosylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| N169 | N-Glycosylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| K218 | Acetylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| N242 | N-Glycosylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , P05129 (PRKCG) | Uniprot |
| S386 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S393 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S397 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) | Uniprot |
| S406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot |
研究背景
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sulfated on tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Secreted>Extracellular space.
Expressed in the retina pigment epithelium (at protein level). Plasma.
Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1, insulin and C1QBP.
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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