SERPINF1 Antibody - #DF6547

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产品: SERPINF1 抗体
货号: DF6547
描述: Rabbit polyclonal antibody to SERPINF1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 46kDa; 46kD(Calculated).
蛋白号: P36955
RRID: AB_2838509

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%)
克隆:
Polyclonal
特异性:
SERPINF1 Antibody detects endogenous levels of total SERPINF1.
RRID:
AB_2838509
引用格式: Affinity Biosciences Cat# DF6547, RRID:AB_2838509.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; OI12; OI6; PEDF; PEDF_HUMAN; PIG 35; PIG35; Pigment epithelium derived factor; Pigment epithelium-derived factor; Proliferation inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Serpin F1; Serpin family F member 1; Serpin peptidase inhibitor clade F member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; SERPINF 1; Serpinf1;

抗原和靶标

免疫原:
Uniprot:

P36955(PEDF_HUMAN) >>Visit HPA database.

基因/基因ID:
SERPINF1(5176)
表达:
P36955 PEDF_HUMAN:

Retinal pigment epithelial cells and blood plasma.

描述:
Pigment epithelium-derived growth factor (PEDF), also known as EPC-1 (early population doubling level cDNA-1), is a glycoprotein found naturally in the normal eye. PEDF has reported neuroprotective and differentiation properties and is secreted in abundance by retinal pigment epithelium cells. It belongs to the serine protease inhibitor (Serpin) superfamily and has been reported to inhibit angiogenesis and proliferation of several cell types. The “pooling” of PEDF within the interphotoreceptor matrix places this molecule in a prime physical location to affect the underlying neural retina. Additionally, PEDF induces neuronal differentiation and promotes survival of neurons of the central nervous system from degeneration caused by serum withdrawal or glutamate cytotoxicity.
序列:
MQALVLLLCIGALLGHSSCQNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
92
Rabbit
92
Chicken
58
Xenopus
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P36955 作为底物

Site PTM Type Enzyme
S24 Phosphorylation P68400 (CSNK2A1)
S114 Phosphorylation P68400 (CSNK2A1)
S152 Phosphorylation
S153 Phosphorylation
S227 Phosphorylation P17612 (PRKACA)
T283 Phosphorylation
N285 N-Glycosylation
T287 Phosphorylation
T294 Phosphorylation
S295 Phosphorylation
K316 Methylation

研究背景

功能:

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

翻译修饰:

The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.

N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

细胞定位:

Secreted. Melanosome.
Note: Enriched in stage I melanosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Retinal pigment epithelial cells and blood plasma.

蛋白家族:

The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.

Belongs to the serpin family.

研究领域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

文献引用

1). PEDF is an antifibrosis factor that inhibits the activation of fibroblasts in a bleomycin-induced pulmonary fibrosis rat model. Respiratory Research, 2022 (PubMed: 35459189) [IF=5.8]

Application: IF/ICC    Species: rat    Sample: lung

Fig. 2 |Angiogenesis and PEDF expression and distribution.F Immunofuorescent staining showed the localization and expression of PEDF in the lung from normal and BLM-exposed (28 days) rats. Bar = 200 μm

Application: IHC    Species: rat    Sample: lung

Fig. 3| PEDF could inhibit fbrosis induced by BLM. A Representative images of the H&E and Masson-stained cross-section of the lung from the experimental group (BLM 5 mg/kg or 2 mg/kg) and PEDF-AAV (denoted as PEDF) treatment group or PEDF-shRNA-AAV (denoted as shPEDF)treatment group. BLM (2 mg/kg or 5 mg/kg) represents lung tissue from rats 28 days after BLM instillation. Bar=200 μm.

Application: WB    Species: rat    Sample: fibroblasts

Fig. 4| PEDF inhibited fbroblast activation by inhibiting the TGF-β1/smad2/3 pathway. D and E Western blot to detect the efect of diferent concentrations of TGF-β1 on PEDF expression.
2). PEDF Protects Endothelial Barrier Integrity during Acute Myocardial Infarction via 67LR. International Journal of Molecular Sciences, 2023 (PubMed: 36769107) [IF=5.6]
3). Preparation and Characterization of Protein-loaded PFC Nanoemulsions for the Treatment of Heart Diseases by Pulmonary Administration. European Journal of Pharmaceutical Sciences, 2021 (PubMed: 33359617) [IF=4.6]
4). A "Hibernating-like" viable state induced by Lentiviral Vector-Mediated PEDF overexpression in rat acute ischemic myocardium. Human Gene Therapy, 2019 (PubMed: 30734585) [IF=4.2]

Application: WB    Species: rat    Sample: cardiomyocytes

Figure 6. |Cardioprotective effect of PEDF in neonatal cardiomyocytes and contractile recoverability of anoxic cardiomyocytes. (A) Representative western blots of PEDF and RIP3 protein in neonatal cardiomyocytes transduced by PEDF-LVs or not and subjected to anoxic insult at 0-12 hours. 0 hour represents normal control. The labels 0 + P, 1 + P, 2 + P, 4 + P, 8 + P, and 12 + P represent relative anoxic period plus PEDF-LVs transduced.

Application: IHC    Species: rat    Sample: myocardium

Figure 5. |Ultrastructure change observed by electron microscopy and glycogen deposition demonstrated by PAS staining. (A) Representative ultrastructure images of myocardium observed by electron microscopy. The black arrow indicates the area of glycogen. The blue arrow indicates the area of mitochondria. The yellow arrow indicates the reserved contractile material (bar = 2 μm). (B) PAS staining images of myocardium observed by light microscopy. The red arrow indicates the area of glycogen (bar = 10 μm). All Biopsy sections were taken from the LAD distribution of the animals sacrificed after PET and echocardiographic studies 3 days after AMI.
5). PEDF inhibits non‑small cell lung cancer proliferation by suppressing autophagy through downregulation of AMPK‑ULK1 signaling. Oncology Reports, 2022 (PubMed: 36281945) [IF=4.2]

Application: WB    Species: Human    Sample: NSCLC cell

Figure 1. Expression of PEDF in normal and cancer tissues and the effect of PEDF expression on cell proliferation. Three types of NSCLC cell lines H460, A549 and H1299 were assessed, and HBE and fibroblast cell lines were used as normal controls. (A and B) WB was used to determine the expression of PEDF in NSCLC cells. Detection of the mRNA expression of PEDF using RT-qPCR. *P

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