产品: OCT1 抗体
货号: DF6553
描述: Rabbit polyclonal antibody to OCT1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 76kDa; 76kD(Calculated).
蛋白号: P14859
RRID: AB_2838515

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
OCT1 Antibody detects endogenous levels of total OCT1.
RRID:
AB_2838515
引用格式: Affinity Biosciences Cat# DF6553, RRID:AB_2838515.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain, class 2, transcription factor 1; POU2F1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P14859 PO2F1_HUMAN:

Ubiquitous. Isoform 2 is lymphocyte-specific.

描述:
Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).
序列:
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14859 作为底物

Site PTM Type Enzyme
S78 Phosphorylation P78527 (PRKDC)
S81 Phosphorylation P78527 (PRKDC)
S85 Phosphorylation P78527 (PRKDC)
S88 Phosphorylation P78527 (PRKDC)
S92 Phosphorylation P78527 (PRKDC)
T100 Phosphorylation P78527 (PRKDC)
S141 Phosphorylation P78527 (PRKDC)
S143 Phosphorylation P78527 (PRKDC)
S147 Phosphorylation P78527 (PRKDC)
T162 Phosphorylation P78527 (PRKDC)
S167 Phosphorylation P78527 (PRKDC)
T226 Phosphorylation P78527 (PRKDC)
S232 Phosphorylation P78527 (PRKDC)
T251 Phosphorylation
T255 Phosphorylation
T259 Phosphorylation
S267 Phosphorylation
S269 Phosphorylation P24941 (CDK2)
T270 Phosphorylation P24941 (CDK2)
K272 Acetylation
K272 Ubiquitination
T276 Phosphorylation
S278 Phosphorylation
S283 Phosphorylation
K293 Ubiquitination
K301 Ubiquitination
K315 Ubiquitination
S335 Phosphorylation Q9HC98 (NEK6) , Q9Y478 (PRKAB1) , Q13131 (PRKAA1)
S366 Phosphorylation
S370 Phosphorylation
S385 Phosphorylation Q13131 (PRKAA1) , P17612 (PRKACA) , P50613 (CDK7)
T445 Phosphorylation
S446 Phosphorylation
S447 Phosphorylation
S448 Phosphorylation

研究背景

功能:

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

翻译修饰:

Phosphorylated by PRKDC.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Isoform 2 is lymphocyte-specific.

亚基结构:

Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.

蛋白家族:

Belongs to the POU transcription factor family. Class-2 subfamily.

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