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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6558, RRID:AB_2838520.
展开/折叠
Factor XII; Beta factor XIIa part 1; Beta factor XIIa part 2; Coagulation factor XII; Coagulation factor XIIa heavy chain; Coagulation factor XIIa light chain; F12; F12 deficiency; FA12_HUMAN; Factor XII deficiency; HAE3; HAEX; HAF; HAF deficiency; Hageman factor;
抗原和靶标
- P00748 FA12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRALLLLGFLLVSLESTLSIPPWEAPKEHKYKAEEHTVVLTVTGEPCHFPFQYHRQLYHKCTHKGRPGPQPWCATTPNFDQDQRWGYCLEPKKVKDHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQKEKCFEPQLLRFFHKNEIWYRTEQAAVARCQCKGPDAHCQRLASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGAFCDVDTKASCYDGRGLSYRGLARTTLSGAPCQPWASEATYRNVTAEQARNWGLGGHAFCRNPDNDIRPWCFVLNRDRLSWEYCDLAQCQTPTQAAPPTPVSPRLHVPLMPAQPAPPKPQPTTRTPPQSQTPGALPAKREQPPSLTRNGPLSCGQRLRKSLSSMTRVVGGLVALRGAHPYIAALYWGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDLTVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPVSYQHDLALLRLQEDADGSCALLSPYVQPVCLPSGAARPSETTLCQVAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00748 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y31 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| T109 | O-Glycosylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| N249 | N-Glycosylation | Uniprot | |
| T299 | O-Glycosylation | Uniprot | |
| T305 | O-Glycosylation | Uniprot | |
| S308 | O-Glycosylation | Uniprot | |
| T328 | O-Glycosylation | Uniprot | |
| T329 | O-Glycosylation | Uniprot | |
| T331 | Phosphorylation | Uniprot | |
| T337 | O-Glycosylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| N433 | N-Glycosylation | Uniprot | |
| R466 | Methylation | Uniprot |
研究背景
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
Secreted.
Interacts with HRG; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding, inhibits factor XII autoactivation and contact-initiated coagulation.
Belongs to the peptidase S1 family.
研究领域
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
文献引用
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