| 产品: | ERCC3 抗体 |
| 货号: | DF6570 |
| 描述: | Rabbit polyclonal antibody to ERCC3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 89kDa; 89kD(Calculated). |
| 蛋白号: | P19447 |
| RRID: | AB_2838532 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6570, RRID:AB_2838532.
展开/折叠
Basic transcription factor 2 89 kDa subunit; BTF 2; BTF2; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; ERCC 3; ercc3; ERCC3_HUMAN; Excision Repair Cross-complementing Rodent Repair deficiency complementation Group 3; GTF2H; RAD 25; RAD25; TFIIH 89 kDa subunit; TFIIH; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; Xeroderma pigmentosum group B-complementing protein;
抗原和靶标
- P19447 ERCC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19447 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y19 | Phosphorylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| K311 | Sumoylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| Y581 | Phosphorylation | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| K706 | Ubiquitination | Uniprot | |
| T720 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y758 | Phosphorylation | Uniprot | |
| K771 | Ubiquitination | Uniprot |
研究背景
ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Nucleus.
Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60. Interacts with ATF7IP. Interacts with KAT2A; leading to KAT2A recruitment to promoters and acetylation of histones.
(Microbial infection) Interacts with Epstein-Barr virus EBNA2.
Belongs to the helicase family. RAD25/XPB subfamily.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
限制条款
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