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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6588, RRID:AB_2838550.
展开/折叠
DNA ligase IV ATP dependent; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG4S; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Sealase;
抗原和靶标
- P49917 DNLI4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49917 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R40 | Methylation | Uniprot | |
| R47 | Methylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S199 | Phosphorylation | Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| Y288 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K432 | Methylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K442 | Methylation | Uniprot | |
| Y454 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K588 | Ubiquitination | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| K645 | Ubiquitination | Uniprot | |
| T650 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S668 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S672 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K710 | Ubiquitination | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| K876 | Ubiquitination | Uniprot | |
| K890 | Ubiquitination | Uniprot |
研究背景
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Nucleus.
Testis, thymus, prostate and heart.
Interacts with XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts with APLF.
Belongs to the ATP-dependent DNA ligase family.
研究领域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
文献引用
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