DNA Ligase IV Antibody - #DF6588

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产品: DNA Ligase IV 抗体
货号: DF6588
描述: Rabbit polyclonal antibody to DNA Ligase IV
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 104kDa; 104kD(Calculated).
蛋白号: P49917
RRID: AB_2838550

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(82%), Zebrafish(82%), Bovine(82%), Horse(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
DNA Ligase IV Antibody detects endogenous levels of total DNA Ligase IV.
RRID:
AB_2838550
引用格式: Affinity Biosciences Cat# DF6588, RRID:AB_2838550.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA ligase IV ATP dependent; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG4S; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Sealase;

抗原和靶标

免疫原:
Uniprot:

P49917(DNLI4_HUMAN) >>Visit HPA database.

基因/基因ID:
LIG4(3981)
表达:
P49917 DNLI4_HUMAN:

Testis, thymus, prostate and heart.

描述:
Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.
序列:
MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Xenopus
91
Rabbit
91
Pig
82
Bovine
82
Zebrafish
82
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P49917 作为底物

Site PTM Type Enzyme
R40 Methylation
R47 Methylation
K55 Ubiquitination
K85 Ubiquitination
K169 Ubiquitination
S199 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S267 Phosphorylation
K273 Ubiquitination
K283 Ubiquitination
Y288 Phosphorylation
K289 Ubiquitination
T347 Phosphorylation
S358 Phosphorylation
T362 Phosphorylation
K376 Ubiquitination
K411 Ubiquitination
K424 Ubiquitination
K432 Methylation
K432 Ubiquitination
K439 Ubiquitination
K442 Methylation
Y454 Phosphorylation
K513 Ubiquitination
K521 Ubiquitination
K571 Ubiquitination
K588 Ubiquitination
K613 Ubiquitination
K645 Ubiquitination
T650 Phosphorylation P78527 (PRKDC)
S668 Phosphorylation P78527 (PRKDC)
S672 Phosphorylation P78527 (PRKDC)
K710 Ubiquitination
K722 Ubiquitination
K733 Ubiquitination
K775 Ubiquitination
K841 Ubiquitination
K876 Ubiquitination
K890 Ubiquitination

研究背景

功能:

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis, thymus, prostate and heart.

亚基结构:

Interacts with XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts with APLF.

蛋白家族:

Belongs to the ATP-dependent DNA ligase family.

研究领域

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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