产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6609, RRID:AB_2838571.
展开/折叠
Beta homolg of S. cerevisiae BUB 1; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles; BUB 1B; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; Bub1A; BUB1B; BUB1B_HUMAN; BUB1beta; BUBR1; Budding Uninhibited by Benzimidazoles 1 beta; Budding uninhibited by benzimidazoles 1 homolog beta (yeast); hBUBR1; MAD3/BUB1 related protein kinase; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint gene BUB1B; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; OTTHUMP00000160319; Protein SSK1; SSK 1; SSK1;
抗原和靶标
Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
- O60566 BUB1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETARQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60566 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K250 | Sumoylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | P33981 (TTK) | Uniprot |
| K438 | Ubiquitination | Uniprot | |
| K451 | Acetylation | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| S543 | Phosphorylation | P06493 (CDK1) , P33981 (TTK) | Uniprot |
| S574 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| T608 | Phosphorylation | Uniprot | |
| T620 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K629 | Ubiquitination | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| Y660 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| K668 | Ubiquitination | Uniprot | |
| S670 | Phosphorylation | P33981 (TTK) , P06493 (CDK1) | Uniprot |
| S676 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T680 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S683 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| K706 | Ubiquitination | Uniprot | |
| T710 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S733 | Phosphorylation | Uniprot | |
| K757 | Ubiquitination | Uniprot | |
| Y766 | Phosphorylation | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| T792 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S797 | Phosphorylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| R919 | Methylation | Uniprot | |
| K988 | Ubiquitination | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| T1008 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K1037 | Ubiquitination | Uniprot | |
| K1040 | Ubiquitination | Uniprot | |
| T1042 | Phosphorylation | Uniprot | |
| S1043 | Phosphorylation | P06493 (CDK1) , P33981 (TTK) | Uniprot |
研究背景
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
Ubiquitinated. Degraded by the proteasome.
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
Cytoplasm. Nucleus. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.
Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with KNL1. Interacts with RIPK3.
The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
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