CTSK Antibody - #DF6614

Figure 1 Histological assays for CTSK and integrin αv in atherosclerosis. (A and D) Typical immunofluorescence image of CTSK and integrin αv in mouse aorta, respectively. (B and E) IHC images of CTSK and integrin αv in mouse aorta, respectively (Designated regions indicated by red square frames in Figures are enlarged to show the details). (C and F) Quantitative IHC analysis of CTSK and integrin αv positive area percentage, respectively (n = 3). Ctrl: control; AS: atherosclerosis.

Fig. 4. DNMT inhibition by SGI-1027 alleviates TP-incurred GPX4 suppression and ferroptotic osteolysis. Mice were allocated to Sham or TP groups (20 mg per mouse, 2 weeks) and treated with control vehicle (Ctrl), SGI-1027 (2.5 mg/kg daily), or Lip-1 (10 mg/kg daily) (n = 6). (A) Exemplary 3D μCT calvarial images and TUNEL-stained calvarial sections. The ROI within μCT images was indicated by squares, while bone resorption areas and TUNEL-positive cells are pointed by arrows. (B) Quantitation of bone porosity and the ratio of TUNEL-positive cells. Data were presented as box-and-whisker plots. *P < 0.05, 2-way ANOVA. The effect size of the interaction between TP-induced calvarial porosity and SGI-1027 (η21) or Lip-1 (η22) intervention was indicated. (C) Western blotting of 2 random calvarial samples from each group for OPN, Col1, CTSK, NFATc1, 4-HNE, and MDA, with β-actin serving as control. (D) Quantification of (C). Data were depicted as mean ± SEM; n = 6; *P < 0.05, 2-way ANOVA.

Figure 4. |Y1R antagonist treatment promoted bone formation and inhibit bone resorption in OVX rats. (A) The Alizarin Red S (yellow arrow head) and TRAP staining (red arrow head) of bone tissues in groups. (B) Immunohistochemical analysis of bone tissue among groups for MMP9 and RUNX2 (x 400). (C, D) Western blotting results of MMP, RUNX2, Cath-K, OPG and RANKL expression in bone marrow from rat femurs. The data are expressed as the means ± SD (n = 6 in each group). ***P<0.001; ****P<0.0001 vs. SHAM, and #P<0.05; ####P<0.0001 vs. OVX by one-way ANOVA and Tukey’s post hoc test

Fig. 5.| SM934-Testosterone suppressed Cathepsin K expression (A) Quantitative real-time PCR analyzed the expression level of Cathepsin K mRNA in MDA-MB231 and SK-BR-3 cells treated with different compounds. (B) Western blot was performed to analyze the expression level of Cathepsin K protein in MDA-MB-231 and SK-BR-3 cells treated with different compounds.

Figure 4 OB abrogates RANKL‐associated NFATc1and c-fos transcription and downregulates osteoclast-related genes. (A) The RT‐qPCR assay detected the relative mRNA expression of osteoclastogenesis-associated marker genes, including NFATc1, c-fos, TRAP, Rank, cathepsin K and MMP9. (B) Western-blot analysis for OB’s effects on protein levels of NFATc1 and c-fos. (C) Western-blot analysis for OB’s effects on protein levels of MMP9 and cathepsin K. (D) Quantitative analysis of NFATc1 and c-fos. (E) Quantitative analysis of MMP9 and cathepsin K. (F) Immunofluorescence images for OB’s effects on protein expression of NFATc1. ns, no statistical significance