产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6617, RRID:AB_2838579.
展开/折叠
DNA directed RNA polymerase II 33 kDa polypeptide; DNA directed RNA polymerase II subunit C; DNA directed RNA polymerase II subunit RPB3; DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; hRPB33; hsRPB3; POLR 2C; POLR2 C; Polr2c; Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase RNA II DNA directed polypeptide C 33kDa; RNA polymerase II polypeptide C; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB 3; RPB3; RPB3_HUMAN; RPB31; RPB33;
抗原和靶标
- P19387 RPB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19387 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T12 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K175 | Sumoylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K205 | Sumoylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| Y220 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Nucleus.
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
研究领域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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