ALDH1A1 Antibody - #DF6625

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产品: ALDH1A1 抗体
货号: DF6625
描述: Rabbit polyclonal antibody to ALDH1A1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 55kDa; 55kD(Calculated).
蛋白号: P00352
RRID: AB_2838587

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(82%), Chicken(82%)
克隆:
Polyclonal
特异性:
ALDH1A1 Antibody detects endogenous levels of total ALDH1A1.
RRID:
AB_2838587
引用格式: Affinity Biosciences Cat# DF6625, RRID:AB_2838587.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acetaldehyde dehydrogenase 1; AHD2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH, liver cytosolic; ALDH-E1; ALDH1 A1; ALDH1; ALDH11; ALDH1A1; ALHDII; cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL-S-53e; MGC2318; PUMB1; RALDH 1; RalDH1; Retinal dehydrogenase 1;

抗原和靶标

免疫原:
Uniprot:

P00352(AL1A1_HUMAN) >>Visit HPA database.

基因/基因ID:
ALDH1A1(216)
描述:
The aldehyde dehydrogenase family is a large group of enzymes that oxidize aldehydes formed through metabolic processes to their carboxylic acids (1). ALDH1A1 is a liver cytosolic isoform of acetaldehyde dehydrogenase and is involved in the major pathway of alcohol metabolism along with alcohol dehydrogenase (2). ALDH1A1 is also known as retinal dehydrogenase 1 and is involved in retinol metabolism, converting retinol to retinoic acid (3). Recent studies suggest that control of retinoid signaling through ALDH1A1 may influence hematopoietic stem cell differentiation (4). There has been recent interest in ALDH1 isoforms as predictive biomarkers in disease. Several studies have suggested that ALDH1A1 is a potential marker for cancer stem cells and chemoresistance in several tumor types, such as melanoma (5), lung cancer (6), glioblastoma (7).
序列:
MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTVKISQKNS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
100
Pig
91
Bovine
91
Sheep
91
Dog
82
Chicken
82
Xenopus
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00352 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T6 Phosphorylation
K22 Acetylation
K65 Acetylation
R68 Methylation
R85 Methylation
K91 Acetylation
T105 Phosphorylation
Y119 Phosphorylation
K128 Acetylation
K128 Ubiquitination
K139 Acetylation
K139 Ubiquitination
T144 Phosphorylation
Y154 Phosphorylation
K252 Acetylation
T267 Phosphorylation
S314 Phosphorylation
Y316 Phosphorylation
T337 Phosphorylation
T341 Phosphorylation
K353 Acetylation
S360 Phosphorylation
K362 Acetylation
K367 Acetylation
K398 Ubiquitination
K410 Acetylation
K412 Acetylation
K412 Ubiquitination
S413 Phosphorylation
K419 Acetylation
T424 Phosphorylation
Y426 Phosphorylation
S429 Phosphorylation
K435 Acetylation
K435 Ubiquitination
T442 Phosphorylation
Y481 Phosphorylation
Y486 Phosphorylation
K490 Ubiquitination
T493 Phosphorylation
K495 Acetylation
K495 Ubiquitination

研究背景

功能:

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo.

翻译修饰:

The N-terminus is blocked most probably by acetylation.

细胞定位:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotetramer.

蛋白家族:

Belongs to the aldehyde dehydrogenase family.

研究领域

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Experimental and Therapeutic Medicine, 2023 (PubMed: 36545274) [IF=2.7]

Application: WB    Species: Human    Sample: SW1990/GZ cells

Figure 2 Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. *P

Application: IHC    Species: Human    Sample: SW1990/GZ cells

Figure 7 Growth of stem cells and EMT progression in SW1990/GZ xenograft model is inhibited by Emo by inactivating Snail. (A) Expression levels of CD44, ALDH1, Nanog and Snail as determined via western blotting. (B) Expression level of CD44, ALDH1, Nanog and Snail in tumor tissues measured via immunohistochemistry (x200 magnification). (C) Expression levels of vimentin and E-cadherin as determined via western blotting. (D) Expression levels of vimentin and E-cadherin in tumor tissues measured via immunohistochemistry (x200 magnification). *P

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