SP-C Antibody - #DF6647

Fig. 1 Single-walled carbon nanotubes (SWCNTs) induced pulmonary fibrosis and senescence of AECIIs in mouse lung tissues. Mice were exposed to 40 μg SWCNT by intratracheal instillation, then lung tissues were collected on days 3, 7 and 28. A Scheme of workflow for evaluation of cellular senescence and pulmonary fibrosis induced by SWCNTs in vivo. B–D Western Blotting analysis of p21 and p16 protein expressions (n = 3) in lung tissues. Contents of TGF-β (E) and PAI-1 (F) in bronchoalveolar lavage fluid (BALF) quantified by ELISA (n = 5). HE staining (G) and Masson’s trichrome staining (H) of mouse lung tissues (200×). I The semiquantitative Ashcroft scores for the severity of pulmonary fibrosis (n = 4). J The hydroxyproline (HYP) level (n = 4) in lung tissues of mice. K Immunohistochemistry (IHC) of COL I expression (n = 5) in lung tissues of mice. L, M The correlation of hydroxyproline contents in mice lung tissues and senescence-associated secretory phenotype (SASP) factors (TGF-β and PAI-1) in BALF. N Immunostaining for p16 (a senescence-related marker) and SP-C (an AECIIs marker) from SWCNTs-exposed lung tissues of mice on day 28 was detected by immunofluorescence (IF) (400×). *P 

Fig. 5 UXM analysis identified various cell populations in EM-seq and were verified by Immunohistochemistry (A) Cell proportion score between tumor (red) and normal (green) tissue analyzed by UXM. (B) The cellular composition of each tumor and normal tissue identified by UXM. (C) Representative immunohistochemistry image shows that CD3、CD19、SP-C were highly expressed in NSCLC tumor tissues (C-E); CD31were highly expressed in normal lung tissue (F); CD56 were weakly expressed in normal lung tissue (G).

Fig. 9 Krt15+ cells transform into AT2 cells to protect alveoli. A Immunofluorescence staining of lung tissue with Krt15 (Red) and Sftpc (Green). B The ratio of Krt15+Sftpc+ cells/Total cells. C Staining-intensity profiles showing signals from three fluorescent channels. D The ratio of TUNEL+ cells/Total cells. E TUNEL staining. N = 3 in each group.*p 

Fig. 2 Effects of RosA on the H1N1 virus-triggered pro-inflammatory response. A Representative immunofluorescence images of IL-6, IL-8 (pink) and TNF-α, MCP-1 (red) in lung SpC+ (green) alveolar epithelial cells. B Quantitative analysis of fluorescence intensities for IL-6, TNF-α, IL-8 and MCP-1 in SpC+ alveolar epithelial cells. C Levels of pro-inflammatory mediators (IL-6, MCP-1, RANTES and TNF-α) in the lung homogenates were determined by Luminex assay. D Levels of pro-inflammatory mediators (IL-6, IL-8, IP-10, MCP-1, RANTES and TNF-α) were determined by Luminex assay.