(1)
(2)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(89%), Zebrafish(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
LIG1 Antibody detects endogenous levels of total LIG1.
RRID:
AB_2838625
引用格式: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
引用格式: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
描述:
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008]
序列:
- P18858 DNLI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Bovine
89
Sheep
89
Zebrafish
89
Xenopus
78
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P18858 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S66 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S76 | Phosphorylation | P06493 (CDK1) , O14757 (CHEK1) , P24941 (CDK2) | Uniprot |
| K79 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T97 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| T171 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T197 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T233 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K244 | Sumoylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| K630 | Sumoylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| T639 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| K795 | Methylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| T798 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| K813 | Ubiquitination | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| Y823 | Phosphorylation | Uniprot | |
| K866 | Ubiquitination | Uniprot | |
| Y897 | Phosphorylation | Uniprot | |
| S911 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| T918 | Phosphorylation | Uniprot | |
| Y919 | Phosphorylation | Uniprot |
研究背景
功能:
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
细胞定位:
Nucleus.
亚基结构:
Interacts with PCNA.
蛋白家族:
Belongs to the ATP-dependent DNA ligase family.
研究领域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Base excision repair.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Replication and repair > Mismatch repair.
文献引用
1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024
(PubMed: 39246208)
[IF=27.4]
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