AREG Antibody - #DF6665

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产品: AREG 抗体
货号: DF6665
描述: Rabbit polyclonal antibody to AREG
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 28kDa; 28kD(Calculated).
蛋白号: P15514
RRID: AB_2838627

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(91%), Dog(92%)
克隆:
Polyclonal
特异性:
AREG Antibody detects endogenous levels of total AREG.
RRID:
AB_2838627
引用格式: Affinity Biosciences Cat# DF6665, RRID:AB_2838627.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A REG; Amphiregulin; Amphiregulin B; AR; AREG; AREG_HUMAN; AREGB; Colorectum cell derived growth factor; Colorectum cell-derived growth factor; CRDGF; MGC13647; Schwannoma derived growth factor; SDGF;

抗原和靶标

免疫原:
Uniprot:

P15514(AREG_HUMAN) >>Visit HPA database.

基因/基因ID:
AREG(374)
描述:
Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs.
序列:
MRAPLLPPAPVVLSLLILGSGHYAAGLDLNDTYSGKREPFSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDNEPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSKIALAAIAAFMSAVILTAVAVITVQLRRQYVRKYEGEAEERKKLRQENGNVHAIA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
91
Pig
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P15514 作为底物

Site PTM Type Enzyme
S41 O-Glycosylation
T52 O-Glycosylation
S53 O-Glycosylation
K109 Ubiquitination
S117 Phosphorylation
K128 Ubiquitination
K129 Ubiquitination
T169 O-Glycosylation
S194 Phosphorylation
K230 Acetylation
K230 Ubiquitination
Y231 Phosphorylation
K239 Acetylation
K240 Acetylation

研究背景

功能:

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

细胞定位:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

The immature precursor interacts with CNIH.

蛋白家族:

Belongs to the amphiregulin family.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

文献引用

1). Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells. Cell reports, 2021 (PubMed: 37060563) [IF=8.8]

Application: IHC    Species: Mouse    Sample:

Figure 5Amphiregulin secretion regulates MAPK signaling

Application: IF/ICC    Species: Mouse    Sample:

Figure 5Amphiregulin secretion regulates MAPK signaling
2). miR-17-5p affects porcine granulosa cell growth and estradiol synthesis by targeting E2F1 gene. REPRODUCTION IN DOMESTIC ANIMALS, 2019 (PubMed: 31424586) [IF=1.7]

Application: WB    Species: porcine    Sample: porcine GCs

FIGURE 3 |miR‐17‐5p up‐regulates LHR, CYP19A1 and AREG expression and oestradiol synthesis in pGCs. All the experiments were done in porcine GCs 48 hr after transfected. (a) The mRNA expression levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by qRT‐PCR. (b) Protein levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by Western blot.

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