(2)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6676, RRID:AB_2838638.
展开/折叠
Bifunctional epoxide hydrolase 2; CEH; Cytosolic epoxide hydrolase; EPHX2; Epoxide hydratase; Epoxide hydrolase 2; Epoxide hydrolase 2 cytoplasmic; epoxide hydrolase 2, cytosolic; Epoxide hydrolase soluble; HYES_HUMAN; SEH; Soluble epoxide hydrolase;
抗原和靶标
- P34913 HYES_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P34913 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K115 | Acetylation | Uniprot | |
| K167 | Acetylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| K478 | Ubiquitination | Uniprot | |
| K483 | Ubiquitination | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K554 | Ubiquitination | Uniprot |
研究背景
Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.
The N-terminus is blocked.
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
Cytoplasm. Peroxisome.
Homodimer.
The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
研究领域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: WB Species: rat Sample: liver
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