产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6677, RRID:AB_2838639.
展开/折叠
D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3_HUMAN; LIG 3; LIG2; LIG3; Ligase II, DNA, ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide synthase [ATP]; Polydeoxyribonucleotide synthase; RP23-316C11.6;
抗原和靶标
- P49916 DNLI3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49916 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K111 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
| T204 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| K213 | Methylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| K316 | Acetylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K615 | Acetylation | Uniprot | |
| K615 | Ubiquitination | Uniprot | |
| T637 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| T647 | Phosphorylation | Uniprot | |
| K660 | Ubiquitination | Uniprot | |
| K663 | Ubiquitination | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| Y666 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K749 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| Y767 | Phosphorylation | Uniprot | |
| Y768 | Phosphorylation | Uniprot | |
| T796 | Phosphorylation | Uniprot | |
| K817 | Methylation | Uniprot | |
| K817 | Sumoylation | Uniprot | |
| K817 | Ubiquitination | Uniprot | |
| K826 | Ubiquitination | Uniprot | |
| K833 | Ubiquitination | Uniprot | |
| S853 | Phosphorylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T877 | Phosphorylation | Uniprot | |
| K883 | Acetylation | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| T910 | Phosphorylation | Uniprot | |
| S912 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | P06493 (CDK1) , P24941 (CDK2) | Uniprot |
研究背景
Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.
Mitochondrion.
Note: Contains an N-terminal mitochondrial transit peptide.
Mitochondrion.
Note: Contains an N-terminal mitochondrial transit peptide.
Nucleus.
Note: Lacks the N-terminal mitochondrial transit peptide.
Nucleus.
Note: Lacks the N-terminal mitochondrial transit peptide.
Testis, thymus, prostate and heart.
Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1.
The PARP-type zinc finger is required for DNA ligase activity.
Belongs to the ATP-dependent DNA ligase family.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
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