产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6690, RRID:AB_2838652.
展开/折叠
MNAT 1; CAP35; CDK activating kinase assembly factor MAT1; CDK-activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; Cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1; MAT1_HUMAN; Menage a trois 1 (CAK assembly factor); Menage a trois; Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis); MNAT CDK activating kinase assembly factor 1; Mnat1; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; TFB3;
抗原和靶标
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
- P51948 MAT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51948 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot |
翻译修饰 - P51948 作为激酶
研究背景
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
Nucleus.
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
限制条款
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