产品: DLC1 抗体
货号: DF6693
描述: Rabbit polyclonal antibody to DLC1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 171kDa; 171kD(Calculated).
蛋白号: Q96QB1
RRID: AB_2838655

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
DLC1 Antibody detects endogenous levels of total DLC1.
RRID:
AB_2838655
引用格式: Affinity Biosciences Cat# DF6693, RRID:AB_2838655.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; DLC1 Rho GTPase activating protein; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer (START) domain containing 12; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q96QB1 RHG07_HUMAN:

Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

描述:
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.
序列:
MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASMEKDATLNVDRKEKCVSLPDCCHGSELRDFPGRPMGHLSKDVDENDSHEGEDQFLSLEASTETLVHVSDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQNEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSTSSSPSGTPTNLRRHVPDLESGSESGADTISVNQTRVNLSSDTESTDLPSSTPVANSGTKPKTTAIQGISEKEKAEIEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELSSFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALNGNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTFNNVVEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENSSDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR

翻译修饰 - Q96QB1 作为底物

Site PTM Type Enzyme
S354 Phosphorylation
S431 Phosphorylation
K513 Sumoylation
S523 Phosphorylation
S526 Phosphorylation
S535 Phosphorylation
S544 Phosphorylation
S548 Phosphorylation
S557 Phosphorylation
S566 Phosphorylation
S577 Phosphorylation
S642 Phosphorylation
S652 Phosphorylation
S673 Phosphorylation
K707 Ubiquitination
S733 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation
T738 Phosphorylation
T740 Phosphorylation
S743 Phosphorylation
S745 Phosphorylation
S747 Phosphorylation
S750 Phosphorylation
T755 Phosphorylation
S757 Phosphorylation
S766 Phosphorylation P31749 (AKT1)
T840 Phosphorylation
S842 Phosphorylation
S850 Phosphorylation
S859 Phosphorylation
S928 Phosphorylation
S946 Phosphorylation
S986 Phosphorylation
S991 Phosphorylation
T993 Phosphorylation
S995 Phosphorylation
S1004 Phosphorylation
K1051 Ubiquitination
S1055 Phosphorylation
S1209 Phosphorylation
T1238 Phosphorylation
S1244 Phosphorylation Q15139 (PRKD1)
S1355 Phosphorylation
T1435 Phosphorylation

研究背景

功能:

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

细胞定位:

Cytoplasm. Cell junction>Focal adhesion. Membrane>Peripheral membrane protein.
Note: Colocalizes with EF1A1 at actin-rich regions in the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

亚基结构:

Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration.

蛋白家族:

The SAM domain mediates interaction with EF1A1, and functions as an autoinhibitory regulator of RhoGAP Activity.

The polybasic cluster is required for activation and mediates binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) containing membranes.

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