GLO1 Antibody - #DF6700

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产品: GLO1 抗体
货号: DF6700
描述: Rabbit polyclonal antibody to GLO1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
分子量: 21kDa; 21kD(Calculated).
蛋白号: Q04760
RRID: AB_2838662

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(83%), Zebrafish(82%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)
克隆:
Polyclonal
特异性:
GLO1 Antibody detects endogenous levels of total GLO1.
RRID:
AB_2838662
引用格式: Affinity Biosciences Cat# DF6700, RRID:AB_2838662.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Aldoketomutase; glo1; GLOD1; Glx I; GLYI; glyoxalase domain containing 1; Glyoxalase I; Ketone aldehyde mutase; Ketone-aldehyde mutase; Lactoyl glutathione lyase; Lactoylglutathione lyase; LGUL_HUMAN; Methylglyoxalase; S D lactoylglutathione methylglyoxal lyase; S-D-lactoylglutathione methylglyoxal lyase;

抗原和靶标

免疫原:
Uniprot:

Q04760(LGUL_HUMAN) >>Visit HPA database.

基因/基因ID:
GLO1(2739)
描述:
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
序列:
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Dog
92
Pig
83
Zebrafish
82
Xenopus
50
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q04760 作为底物

Site PTM Type Enzyme
A2 Acetylation
T35 Phosphorylation
R38 Methylation
K44 Ubiquitination
S45 Phosphorylation
K60 Ubiquitination
C61 S-Nitrosylation
K88 Ubiquitination
T107 Phosphorylation Q9UQM7 (CAMK2A)
Y136 Phosphorylation
S137 Phosphorylation
C139 S-Nitrosylation
K140 Acetylation
K140 Ubiquitination
K148 Acetylation
K148 Ubiquitination
K151 Ubiquitination
K157 Acetylation
K159 Acetylation

研究背景

功能:

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.

翻译修饰:

Glutathionylation at Cys-139 inhibits enzyme activity.

Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B.

Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the glyoxalase I family.

研究领域

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

文献引用

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports, 2021 (PubMed: 34490476) [IF=3.4]

Application: WB    Species: Mouse    Sample: MPC5 cells

Figure 1. AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase-1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH. (B) MPC5 cells were transfected with siRNA NC, siRNA AR or PFKFB3 siRNA1, −2 or −3 for 24 h. Then, the protein expression levels of PFKFB3 and AR were measured by western blot analysis and normalized to GAPDH. **P<0.01 vs. Control (NG). AR, aldose reductase; HG, high glucose; NC, negative control; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; siRNA, small interfering RNA.

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 1.| AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase‑1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH.

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