| 产品: | GRIK2 抗体 |
| 货号: | DF6706 |
| 描述: | Rabbit polyclonal antibody to GRIK2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 103kDa; 103kD(Calculated). |
| 蛋白号: | Q13002 |
| RRID: | AB_2838668 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6706, RRID:AB_2838668.
展开/折叠
bA487F5.1; EAA4; Excitatory amino acid receptor 4; GLR 6; GLR6; GluK2; GLUK6; GLUR 6; GluR-6; GluR6; Glutamate receptor 6; Glutamate receptor; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; Glutamate receptor ionotropic kainate 2; GRIK 2; GRIK2; GRIK2 protein; GRIK2_HUMAN; ionotropic kainate 2; MRT6;
抗原和靶标
- Q13002 GRIK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKIIFPILSNPVFRRTVKLLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKISTYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVGEFLYKSKKNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13002 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T171 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K212 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| T437 | Phosphorylation | Uniprot | |
| Y590 | Phosphorylation | P12931 (SRC) | Uniprot |
| S666 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| Y702 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S834 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| S868 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K886 | Sumoylation | Uniprot |
研究背景
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 (By similarity).
Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1 (By similarity).
Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome (By similarity).
Phosphorylated by PKC at Ser-868 upon agonist activation, this directly enhance sumoylation.
Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.
Expression is higher in cerebellum than in cerebral cortex.
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers (Probable). Assembles into a kainate-gated homomeric channel that does not bind AMPA. GRIK2 associated to GRIK5 forms functional channels that can be gated by AMPA (By similarity). Interacts with DLG4. Interacts with NETO2 (By similarity). Interacts (via C-terminus) with KLHL17 (via kelch repeats); the interaction targets GRIK2 for degradation via ubiquitin-proteasome pathway (By similarity).
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Nervous system > Glutamatergic synapse.
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