ODC1 Antibody - #DF6712

Fig. 3 The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus. (a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV's levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malignant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control.

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control. B, Relative intensity of ODC and ARG1 protein. C, Immunofluorescence staining for IL-33–induced M2 macrophages. The green signal represents the staining of activated ODC, and the blue signal represents the DAPI-stained nuclei (scale bar = 20 μm). *P < .05; **P < .01; ***P < .001. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel

Figure 5 The effects of different concentrations of UA alone or in combination with Dox on ODC and phosphorylation of Erk1/2, VEGF, MMP-9. (A): representative immunoreactive bands of ODC, VEGF, MMP-9, GAPDH, total and phosphorylated Erk1/2 using specific antibody. (B–E): representative quantification of ODC, phosphorylated Erk1/2, VEGF, MMP-9. (F): determination of MMP-9 activity. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with MCF-7/ADR cells, ## p < 0.01, ### p < 0.001, comparison between MCF-7/ADR group and MCF-7 group.

Figure 3. IHC staining showed the up-regulated arginine transporter (CAT1), down-regulated succinic acid synthetase1 (ASS1) in ESCC, and up-regulated ornithine decarboxylase (ODC1) in ESCC (A) The bar graph of the IHC score of the CAT1 (A), ASS1 (B), and ODC1 (C) between cancer and normal tissue, and representative images of IHC staining at a magnification of 40x and 200x.