产品: | MCM6 抗体 |
货号: | DF6716 |
描述: | Rabbit polyclonal antibody to MCM6 |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Horse, Sheep, Rabbit |
分子量: | 93kDa, 120 kDa; 93kD(Calculated). |
蛋白号: | Q14566 |
RRID: | AB_2838678 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6716, RRID:AB_2838678.
展开/折叠
DNA replication licensing factor MCM6; MCG40308; Mcm6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); MCM6 minichromosome maintenance deficient 6; MCM6_HUMAN; Minichromosome maintenance complex component 6; Minichromosome maintenance deficient (mis5 S. pombe) 6; Minichromosome maintenance deficient 6 homolog; Minichromosome maintenance protein 6; Mis5; MIS5 homolog; p105MCM;
抗原和靶标
- Q14566 MCM6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14566 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
K28 | Ubiquitination | Uniprot | |
K95 | Ubiquitination | Uniprot | |
K102 | Ubiquitination | Uniprot | |
K108 | Ubiquitination | Uniprot | |
Y111 | Phosphorylation | Uniprot | |
T119 | Phosphorylation | Uniprot | |
T127 | Phosphorylation | Uniprot | |
S153 | Phosphorylation | Uniprot | |
K173 | Ubiquitination | Uniprot | |
T195 | Phosphorylation | Uniprot | |
K197 | Ubiquitination | Uniprot | |
K205 | Methylation | Uniprot | |
K205 | Ubiquitination | Uniprot | |
R217 | Methylation | Uniprot | |
S219 | Phosphorylation | Uniprot | |
K241 | Ubiquitination | Uniprot | |
K256 | Ubiquitination | Uniprot | |
S258 | Phosphorylation | Uniprot | |
T259 | Phosphorylation | Uniprot | |
T266 | Phosphorylation | Uniprot | |
S271 | Phosphorylation | Uniprot | |
Y276 | Phosphorylation | Uniprot | |
T278 | Phosphorylation | Uniprot | |
R282 | Methylation | Uniprot | |
Y294 | Phosphorylation | Uniprot | |
K313 | Acetylation | Uniprot | |
K313 | Ubiquitination | Uniprot | |
R316 | Methylation | Uniprot | |
S324 | Phosphorylation | Uniprot | |
K326 | Ubiquitination | Uniprot | |
K332 | Ubiquitination | Uniprot | |
K336 | Ubiquitination | Uniprot | |
K344 | Acetylation | Uniprot | |
K344 | Ubiquitination | Uniprot | |
K365 | Ubiquitination | Uniprot | |
T380 | Phosphorylation | Uniprot | |
S385 | Phosphorylation | Uniprot | |
K402 | Ubiquitination | Uniprot | |
K407 | Ubiquitination | Uniprot | |
S413 | Phosphorylation | Uniprot | |
K422 | Ubiquitination | Uniprot | |
S424 | Phosphorylation | Uniprot | |
S425 | Phosphorylation | Uniprot | |
K486 | Ubiquitination | Uniprot | |
K490 | Ubiquitination | Uniprot | |
S507 | Phosphorylation | Uniprot | |
K517 | Acetylation | Uniprot | |
K517 | Ubiquitination | Uniprot | |
Y546 | Phosphorylation | Uniprot | |
S557 | Phosphorylation | Uniprot | |
R565 | Methylation | Uniprot | |
S568 | Phosphorylation | Uniprot | |
R573 | Methylation | Uniprot | |
K588 | Ubiquitination | Uniprot | |
S590 | Phosphorylation | Uniprot | |
K599 | Acetylation | Uniprot | |
K599 | Ubiquitination | Uniprot | |
K611 | Ubiquitination | Uniprot | |
T617 | Phosphorylation | Uniprot | |
K643 | Acetylation | Uniprot | |
K643 | Ubiquitination | Uniprot | |
K646 | Acetylation | Uniprot | |
K646 | Ubiquitination | Uniprot | |
K654 | Ubiquitination | Uniprot | |
T661 | Phosphorylation | Uniprot | |
S689 | Phosphorylation | Uniprot | |
S718 | Phosphorylation | Uniprot | |
Y720 | Phosphorylation | Uniprot | |
K733 | Ubiquitination | Uniprot | |
K744 | Ubiquitination | Uniprot | |
K754 | Ubiquitination | Uniprot | |
S758 | Phosphorylation | Uniprot | |
S762 | Phosphorylation | Uniprot | |
K769 | Ubiquitination | Uniprot | |
K775 | Acetylation | Uniprot | |
K775 | Ubiquitination | Uniprot | |
T791 | Phosphorylation | Uniprot | |
K796 | Ubiquitination | Uniprot | |
Y810 | Phosphorylation | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Nucleus.
Note: Binds to chromatin during G1 and detach from it during S phase.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). May interact with MCM10. Interacts with TIPIN. Interacts with CDT1. Interacts with MCMBP. Interacts with DDI2.
Belongs to the MCM family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
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