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产品:	PGK1 抗体
货号:	DF6722
描述:	Rabbit polyclonal antibody to PGK1
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	45kDa; 45kD(Calculated).
蛋白号:	P00558
RRID:	AB_2838684

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)

克隆:

Polyclonal

特异性:

PGK1 Antibody detects endogenous levels of total PGK1.

RRID:

AB_2838684
引用格式: Affinity Biosciences Cat# DF6722, RRID:AB_2838684.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2;

抗原和靶标

免疫原:

Uniprot:

P00558(PGK1_HUMAN) >>Visit HPA database.

基因/基因ID:

PGK1(5230)

表达:

P00558 PGK1_HUMAN:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

描述:

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

序列:

P00558 PGK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Rabbit

100

Horse

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00558 作为底物

P00558

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
S4	Phosphorylation	Uniprot
K6	Acetylation	Uniprot
K6	Ubiquitination	Uniprot
K11	Acetylation	Uniprot
K11	Ubiquitination	Uniprot
K15	Ubiquitination	Uniprot
K17	Ubiquitination	Uniprot
K30	Acetylation	Uniprot
K30	Methylation	Uniprot
K30	Sumoylation	Uniprot
K30	Ubiquitination	Uniprot
T35	Phosphorylation	Uniprot
R39	Methylation	Uniprot
K41	Ubiquitination	Uniprot
S46	Phosphorylation	Uniprot
K48	Acetylation	Uniprot
K48	Ubiquitination	Uniprot
C50	S-Nitrosylation	Uniprot
K56	Acetylation	Uniprot
K56	Ubiquitination	Uniprot
K75	Acetylation	Uniprot
K75	Ubiquitination	Uniprot
Y76	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
K86	Acetylation	Uniprot
K86	Ubiquitination	Uniprot
S87	Phosphorylation	Uniprot
K91	Acetylation	Uniprot
K91	Ubiquitination	Uniprot
K97	Acetylation	Uniprot
K97	Ubiquitination	Uniprot
C99	S-Nitrosylation	Uniprot
K106	Acetylation	Uniprot
K106	Ubiquitination	Uniprot
C108	S-Nitrosylation	Uniprot
S115	Phosphorylation	Uniprot
R123	Methylation	Uniprot
K131	Acetylation	Uniprot
K131	Ubiquitination	Uniprot
K133	Acetylation	Uniprot
S136	Phosphorylation	Uniprot
K139	Ubiquitination	Uniprot
K141	Acetylation	Uniprot
K141	Ubiquitination	Uniprot
K146	Acetylation	Uniprot
K146	Ubiquitination	Uniprot
S153	Phosphorylation	Uniprot
S155	Phosphorylation	Uniprot
K156	Acetylation	Uniprot
K156	Ubiquitination	Uniprot
Y161	Phosphorylation	Uniprot
T168	Phosphorylation	Uniprot
S175	Phosphorylation	Uniprot
K184	Ubiquitination	Uniprot
K191	Acetylation	Uniprot
K191	Methylation	Uniprot
K191	Ubiquitination	Uniprot
K192	Ubiquitination	Uniprot
Y196	Phosphorylation	Uniprot
K199	Acetylation	Uniprot
K199	Ubiquitination	Uniprot
S203	Phosphorylation	Uniprot
K216	Acetylation	Uniprot
K216	Ubiquitination	Uniprot
K220	Acetylation	Uniprot
K220	Ubiquitination	Uniprot
T243	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
K264	Acetylation	Uniprot
K264	Ubiquitination	Uniprot
K267	Acetylation	Uniprot
K267	Ubiquitination	Uniprot
S271	Phosphorylation	Uniprot
K272	Acetylation	Uniprot
K272	Ubiquitination	Uniprot
K275	Acetylation	Uniprot
K279	Ubiquitination	Uniprot
T281	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
K291	Acetylation	Uniprot
K291	Ubiquitination	Uniprot
K297	Ubiquitination	Uniprot
S305	Phosphorylation	Uniprot
K322	Acetylation	Uniprot
K322	Ubiquitination	Uniprot
K323	Acetylation	Uniprot
K323	Ubiquitination	Uniprot
K332	Ubiquitination	Uniprot
K353	Acetylation	Uniprot
K353	Ubiquitination	Uniprot
K361	Acetylation	Uniprot
K361	Ubiquitination	Uniprot
R365	Methylation	Uniprot
C367	S-Nitrosylation	Uniprot
T378	Phosphorylation	Uniprot
C379	S-Nitrosylation	Uniprot
C380	S-Nitrosylation	Uniprot
K382	Acetylation	Uniprot
K382	Ubiquitination	Uniprot
K388	Acetylation	Uniprot
K388	Ubiquitination	Uniprot
S399	Phosphorylation	Uniprot
K406	Acetylation	Uniprot
K406	Ubiquitination	Uniprot
S415	Phosphorylation	Uniprot

翻译修饰 - P00558 作为激酶

P00558

Substrate	Site	Source
Q15118 (PDK1)	T338	Uniprot

研究背景

P00558_PGK1_HUMAN

功能:

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

细胞定位:

Cytoplasm.

组织特异性:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

亚基结构:

Monomer.

蛋白家族:

Belongs to the phosphoglycerate kinase family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. Heliyon, 2023 (PubMed: 36755606) [IF=4.0]

Application: IHC Species: Human Sample: HER2+ BRCA tissues

Fig. 2 (A) Enrichment of glycolysis-related genes was significant in S100A9 positive BRCA cases (NES > 1, FDR q-value < 0.001). (B) S100A9 silencing impaired the expression of PGK1, LDHA, and ENO1 in both SK-BR-3 and BT474 cell lines. (C) IHC staining results of HER2+ BRCA tissues confirmed the upregulation of PGK1, LDHA, and ENO1 in S100A9 abundant cases (Scale bar = 100 μm, 200 μm, and 400 μm). (D) ECAR level significantly declined when S100A9 was absent from the SK-BR-3 and BT474 cell lines. (E) lactate production and glucose consumption level significantly declined when S100A9 was absent from the SK-BR-3 and BT474 cell lines. PGK1: Phosphoglycerate kinase 1. LDHA: Lactate dehydrogenase A. ENO1: Enolase α. ECAR: Extracellular acidification rate. S100A9: S100 calcium-binding protein A9. BRCA: Breast cancer. HER2: Human epidermal growth factor receptor 2. NES: Normalised enrichment score. FDR: False discovery rate. IHC:Immunohistochemical staining.

2). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.9]

Application: WB Species: Human Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.

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PGK1 Antibody - #DF6722

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