产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6723, RRID:AB_2838685.
展开/折叠
NGS 17; NGS-17; NGS17; TAP associated protein; TAP binding protein; TAP binding protein (tapasin); TAP BP; TAP-associated protein; TAP-binding protein; TAPA; Tapasin; TAPBP; TPN; TPSN; TPSN_HUMAN;
抗原和靶标
- O15533 TPSN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHDPAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLRPQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQHLGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPRVQPFQEGTYLATIHLPYLQGQVTLELAVYKPPKVSLMPATLARAAPGEAPPELLCLVSHFYPSGGLEVEWELRGGPGGRSQKAEGQRWLSALRHHSDGSVSLSGHLQPPPVTTEQHGARYACRIHHPSLPASGRSAEVTLEVAGLSGPSLEDSVGLFLSAFLLLGLFKALGWAAVYLSTCKDSKKKAE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15533 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| N253 | N-Glycosylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K441 | Acetylation | Uniprot | |
| K445 | Acetylation | Uniprot |
研究背景
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Endoplasmic reticulum membrane>Single-pass type I membrane protein.
Neutrophils, mostly in fully differentiated cells.
Heterodimer with PDIA3; disulfide-linked. Interacts with TAP1 and is thus a subunit of the TAP complex, also known as the peptide-loading complex (PLC). Interaction with TAP1 is TAP2-independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP. Interacts with HLA-G-B2M complex; this interaction is required for loading of high affinity peptides.
The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region.
研究领域
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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