HSD17B2 Antibody - #DF6734

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产品: HSD17B2 抗体
货号: DF6734
描述: Rabbit polyclonal antibody to HSD17B2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Dog
分子量: 42kDa; 43kD(Calculated).
蛋白号: P37059
RRID: AB_2838696

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Dog(100%)
克隆:
Polyclonal
特异性:
HSD17B2 Antibody detects endogenous levels of total HSD17B2.
RRID:
AB_2838696
引用格式: Affinity Biosciences Cat# DF6734, RRID:AB_2838696.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

17 beta HSD 2; 17-beta-HSD 2; 17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha HSD; 20 alpha hydroxysteroid dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; DHB2_HUMAN; E2DH; EDH17B2; Estradiol 17-beta-dehydrogenase 2; HSD17; Hsd17b2; Hydroxysteroid (17 beta) dehydrogenase 2; Microsomal 17 beta hydroxysteroid dehydrogenase; Microsomal 17-beta-hydroxysteroid dehydrogenase; SDR9C2; Short chain dehydrogenase/reductase family 9C, member 2; Testosterone 17 beta dehydrogenase; Testosterone 17-beta-dehydrogenase;

抗原和靶标

免疫原:
Uniprot:

P37059(DHB2_HUMAN) >>Visit HPA database.

基因/基因ID:
HSD17B2(3294)
序列:
MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
100
Xenopus
60
Pig
0
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P37059 作为底物

Site PTM Type Enzyme
K141 Acetylation
S251 Phosphorylation
S259 Phosphorylation
T267 Phosphorylation
S273 Phosphorylation

研究背景

功能:

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

细胞定位:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

研究领域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

文献引用

1). Single-cell RNA sequencing reveals that HSD17B2 in cancer-associated fibroblasts promotes the development and progression of castration-resistant prostate cancer. Cancer Letters, 2023 (PubMed: 37244445) [IF=9.7]
2). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: WB    Species: Human    Sample: LC540 (17β-HSD3) cells

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.
3). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]

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