产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6737, RRID:AB_2838699.
展开/折叠
26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Human immunodeficiency virus tat transactivator binding protein 1; MGC8487; Proteasome (prosome macropain) 26S subunit ATPase 3; Proteasome 26S ATPase subunit 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; PRS6A_HUMAN; PSMC 3; PSMC3; Tat binding protein 1; TAT-binding protein 1; TBP-1; TBP1;
抗原和靶标
- P17980 PRS6A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARACAAQTKATFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPSIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLDGFQPNTQVKVIAATNRVDILDPALLRSGRLDRKIEFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYYA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17980 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| R61 | Methylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T131 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | Uniprot | |
| C387 | S-Nitrosylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
Cytoplasm. Nucleus.
Note: Colocalizes with TRIM5 in the cytoplasmic bodies.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC3 and few additional components. Interacts with PAAF1.
(Microbial infection) Interacts with HIV-1 Tat.
Belongs to the AAA ATPase family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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