| 产品: | TAF9 抗体 |
| 货号: | DF6757 |
| 描述: | Rabbit polyclonal antibody to TAF9 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Dog |
| 分子量: | 20kDa; 29kD(Calculated). |
| 蛋白号: | Q16594 |
| RRID: | AB_2838719 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6757, RRID:AB_2838719.
展开/折叠
TAF9 RNA polymerase II, TATA box-binding protein-associated factor, 32kDa; MGC:1603; MGC:3647; MGC:5067; RNA polymerase II TBP-associated factor subunit G; STAF31/32; TAF17; TAF2G; TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa; TAFII31; TAFII32; TAFIID32; TATA box-binding protein-associated factor 2G; TBP associated factor 17 kDa; TBP associated factor 9; TBP associated factor, RNA polymerase II, 32-KD; transcription initiation factor TFIID 31 kD subunit; transcription initiation factor TFIID 31 kDa subunit; transcription initiation factor TFIID 32 kDa subunit; Transcription initiation factor TFIID subunit 9;
抗原和靶标
- Q16594 TAF9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESGKTASPKSMPKDAQMMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKATVDADDVRLAIQCRADQSFTSPPPRDFLLDIARQRNQTPLPLIKPYSGPRLPPDRYCLTAPNYRLKSLQKKASTSAGRITVPRLSVGSVTSRPSTPTLGTPTPQTMSVSTKVGTPMSLTGQRFTVQMPTSQSPAVKASIPATSAVQNVLINPSLIGSKNILITTNMMSSQNTANESSNALKRKREDDDDDDDDDDDYDNL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16594 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Acetylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y57 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Methylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| R119 | Methylation | Uniprot | |
| R128 | Methylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| R186 | Methylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K245 | Sumoylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| Y261 | Phosphorylation | Uniprot |
研究背景
Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.
Nucleus.
Component of TFIID, the TATA-binding protein-free TAF complex (TFTC), the PCAF complex and the STAGA transcription coactivator-HAT complex. The PCAF complex consists at least of TADA2L/ADA2, SUPT3H/SPT3, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. The STAGA transcription coactivator-HAT complex consists at least of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Binds N-terminal domain of p53/TP53 which is essential for transcription. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Binds TFIIB and the Herpes simplex virus activator VP16. Forms a heterodimer with TAF6/TAFII80 in a complex with the TAF4B/TAFII105-TAF12/TAFII20 heterodimer. Also interacts with TAF5. Binds directly DNA. Increased DNA binding when complexed with TAF6/TAFII80.
Belongs to the TAF9 family.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
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