产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6771, RRID:AB_2838733.
展开/折叠
CD111; CD111 antigen; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig like receptor; Herpesvirus Ig-like receptor; HIgR; HveC; MGC142031; Nectin 1; Nectin-1; OFC7; OROFACIAL CLEFT 7; OTTHUMP00000232093; OTTHUMP00000232094; OTTHUMP00000232095; Poliovirus receptor related protein 1; poliovirus receptor-like 1; Poliovirus receptor-related protein 1; PRR; PRR1; PVRL 1; PVRL1; PVRL1_HUMAN; PVRR; PVRR1; SK-12;
抗原和靶标
- Q15223 NECT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARMGLAGAAGRWWGLALGLTAFFLPGVHSQVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAKKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPSPPEHGRRAGPVPTAIIGGVAGSILLVLIVVGGIVVALRRRRHTFKGDYSTKKHVYGNGYSKAGIPQHHPPMAQNLQYPDDSDDEKKAGPLGGSSYEEEEEEEEGGGGGERKVGGPHPKYDEDAKRPYFTVDEAEARQDGYGDRTLGYQYDPEQLDLAENMVSQNDGSFISKKEWYV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15223 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S52 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| N72 | N-Glycosylation | Uniprot | |
| N139 | N-Glycosylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| N202 | N-Glycosylation | Uniprot | |
| Y211 | Phosphorylation | Uniprot | |
| N332 | N-Glycosylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| T391 | Phosphorylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| Y400 | Phosphorylation | Uniprot | |
| Y418 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| Y481 | Phosphorylation | Uniprot | |
| Y488 | Phosphorylation | Uniprot | |
| Y490 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot |
研究背景
Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity.
(Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV.
Cell membrane>Single-pass type I membrane protein. Cell junction>Synapse>Presynaptic cell membrane.
Cell membrane>Single-pass type I membrane protein.
Secreted.
Interacts (via Ig-like C2-type domain 2) with FGFR1, FGFR2 and FGFR3 (By similarity). Cis- and trans-homodimer. Can form trans-heterodimers with NECTIN3 and with NECTIN4. Interaction between NECTIN1 and NECTIN3 on the pre- and postsynaptic sites, respectively, initiates the formation of puncta adherentia junctions between axons and dendrites. Interacts (via cytoplasmic domain) with AFDN (via PDZ domain); this interaction recruits NECTIN1 to cadherin-based adherens junctions and provides a connection with the actin cytoskeleton. Interacts with integrin alphaV/beta3.
(Microbial infection) Interacts with herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV envelope glycoprotein D.
Ig-like C2-type 2 mediates neurite outgrowth through binding, induction of phosphorylation, and activation of FGFR.
Belongs to the nectin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
限制条款
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