产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6788, RRID:AB_2838750.
展开/折叠
4930549J05Rik; A430104F18Rik; AW552088; CD247; CD247 antigen; Cd247 molecule; Cd3; CD3 antigen, zeta polypeptide, isoform CRA_b; CD3 antigen, zeta subunit; CD3-eta; CD3H; CD3Q; Cd3z; CD3Z antigen zeta polypeptide (TiT3 complex); CD3Z_HUMAN; CD3zeta; CD3zeta chain; MGC140430; T cell receptor T3 zeta chain; T cell surface glycoprotein CD3 zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; T3z; TCR zeta chain; TCRk; TCRZ; TCRzeta;
抗原和靶标
CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).
- P20963 CD3Z_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKWKALFTAAILQAQLPITEAQSFGLLDPKLCYLLDGILFIYGVILTALFLRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P20963 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S58 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | P06241 (FYN) | Uniprot |
| K116 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y123 | Phosphorylation | P06241 (FYN) | Uniprot |
| S124 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| Y142 | Phosphorylation | P06239 (LCK) | Uniprot |
| S146 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot |
研究背景
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Membrane>Single-pass type I membrane protein.
CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs).
The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with SLA. Interacts with TRAT1. Interacts with DOCK2. Interacts with SLA2. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC. Interacts with CRK; this interaction regulates CD3Z phosphorylation. Interacts (on T cell side) with CD81, ICAM1 and CD9 at immunological synapses between antigen-presenting cells and T cells. Interacts with CD160.
(Microbial infection) Interacts with HIV-1 Nef; this interaction up-regulates the expression of the Fas ligand (FASLG) at the cell surface.
(Microbial infection) Interacts with HIV-2 Nef protein; this interaction induces down-regulation of cell surface TCR/CD3 complexes.
The ITAM domains mediate interaction with SHB.
Belongs to the CD3Z/FCER1G family.
研究领域
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
限制条款
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