产品: PPA1 抗体
货号: DF6832
描述: Rabbit polyclonal antibody to PPA1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 33kDa; 33kD(Calculated).
蛋白号: Q15181
RRID: AB_2838791

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(91%)
克隆:
Polyclonal
特异性:
PPA1 Antibody detects endogenous levels of total PPA1.
RRID:
AB_2838791
引用格式: Affinity Biosciences Cat# DF6832, RRID:AB_2838791.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cytosolic inorganic pyrophosphatase; diphosphate phosphohydrolase; inorganic diphosphatase; inorganic pyrophosphatase 1; Inorganic pyrophosphatase; IOPPP; IPYR_HUMAN; PP; PP1; PPA1; PPase; Pyp; pyrophosphatase, inorganic, 1; Pyrophosphate phospho hydrolase; Pyrophosphate phospho-hydrolase; SID6-8061;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q15181 IPYR_HUMAN:

Expressed ubiquitously.

描述:
PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).This antibody is specific to PPA1.
序列:
MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Pig
92
Horse
92
Dog
92
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15181 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S5 Phosphorylation
T6 Phosphorylation
S14 Phosphorylation
Y17 Phosphorylation
K22 Ubiquitination
K25 Acetylation
K25 Ubiquitination
Y28 Phosphorylation
S30 Phosphorylation
Y38 Phosphorylation
K57 Acetylation
K63 Acetylation
K63 Ubiquitination
K70 Acetylation
K70 Ubiquitination
K74 Acetylation
K75 Acetylation
Y80 Phosphorylation
Y94 Phosphorylation
K109 Ubiquitination
K128 Ubiquitination
R132 Methylation
K155 Acetylation
K176 Acetylation
K176 Ubiquitination
K179 Ubiquitination
Y182 Phosphorylation
T186 Phosphorylation
Y193 Phosphorylation
K194 Acetylation
K199 Acetylation
K213 Ubiquitination
K228 Acetylation
K228 Ubiquitination
T235 Phosphorylation
K238 Ubiquitination
S241 Phosphorylation
T246 Phosphorylation
S248 Phosphorylation
S250 Phosphorylation
K253 Acetylation
K253 Ubiquitination
C270 S-Nitrosylation
C274 S-Nitrosylation
K282 Ubiquitination

研究背景

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed ubiquitously.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the PPase family.

研究领域

· Metabolism > Energy metabolism > Oxidative phosphorylation.

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