| 产品: | PPP4C 抗体 |
| 货号: | DF6833 |
| 描述: | Rabbit polyclonal antibody to PPP4C |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 35kDa; 35kD(Calculated). |
| 蛋白号: | P60510 |
| RRID: | AB_2838792 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6833, RRID:AB_2838792.
展开/折叠
PP X; PP-X; Pp4; PP4C; PP4C_HUMAN; PPH3; PPP4; ppp4c; PPX; protein phosphatase 4 (formerly X), catalytic subunit; Protein phosphatase 4 catalytic subunit; Protein phosphatase X; protein phosphatase X, catalytic subunit; Serine/threonine protein phosphatase 4 catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit;
抗原和靶标
- P60510 PP4C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P60510 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R15 | Methylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| R107 | Methylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot |
研究背景
Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 and GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3.
Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
研究领域
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
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