(1)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6863, RRID:AB_2838822.
展开/折叠
CAG 3A; CAG3A; CTG 3a; CTG3a; NBL; NUMB Drosophilia Homolog Like; Numb homolog (Drosophila) like; Numb homolog like; Numb like protein; NUMB R; Numb-like protein; Numb-R; Numb-related gene; Numb-related protein; NUMBL; NUMBL_HUMAN; NUMBLIKE; NUMBR; TNRC 23; TNRC23; Trinucleotide repeat-containing gene 23;
抗原和靶标
- Q9Y6R0 NUMBL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSAAASGGPRRPERHLPPAPCGAPGPPETCRTEPDGAGTMNKLRQSLRRRKPAYVPEASRPHQWQADEDAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQTIEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTAAFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPTVAPGPAQPGHVSPTPATTSPGEKGEAGTPVAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKRQLSLRLNELPSTLQRRTDFQVKGTVPEMEPPGAGDSDSINALCTQISSSFASAGAPAPGPPPATTGTSAWGEPSVPPAAAFQPGHKRTPSEAERWLEEVSQVAKAQQQQQQQQQQQQQQQQQQQQAASVAPVPTMPPALQPFPAPVGPFDAAPAQVAVFLPPPHMQPPFVPAYPGLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPATLLGKAGAFPPPAIPSAPGSQARPRPNGAPWPPEPAPAPAPELDPFEAQWAALEGKATVEKPSNPFSGDLQKTFEIEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6R0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S48 | Phosphorylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| R307 | Methylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot |
研究背景
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
Cytoplasm.
Note: Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.
Interacts (via PTB domain) with MAP3K7IP2 (via C-terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.
The PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
研究领域
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
文献引用
Application: WB Species: Human Sample: Cartilage
限制条款
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