产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6916, RRID:AB_2838875.
展开/折叠
C358B7.1; p18; SUMO 1 protein ligase; SUMO conjugating enzyme UBC9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO1 protein ligase; UBC9; UBC9_HUMAN; UBCE9; Ube2i; Ubiquitin carrier protein 9; Ubiquitin carrier protein; Ubiquitin carrier protein I; Ubiquitin conjugating enzyme 9; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast); Ubiquitin conjugating enzyme UbcE2A; Ubiquitin like protein SUMO 1 conjugating enzyme; Ubiquitin protein ligase E2I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I;
抗原和靶标
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
- P63279 UBC9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P63279 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K14 | Sumoylation | Uniprot | |
| K18 | Sumoylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| K48 | Sumoylation | Uniprot | |
| K49 | Sumoylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K65 | Acetylation | Uniprot | |
| K65 | Sumoylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K74 | Ubiquitination | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K153 | Sumoylation | Uniprot | |
| K154 | Sumoylation | Uniprot |
研究背景
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Nucleus. Cytoplasm.
Note: Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Interacts with SETX. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3. Interacts with NR2C1; the interaction promotes its sumoylation (By similarity). Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML-RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Interacts with FHIT. Interacts with PRKRA and p53/TP53 (By similarity). Interacts with UHRF2. Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1. Interacts with ZNF451. Identified in a complex with SUMO2 and UBE2I, where one ZNF451 interacts with one UBE2I and two SUMO2 chains, one bound to the UBE2I active site and the other to another region of the same UBE2I molecule. Interacts with CPEB3 (By similarity). Interacts with SUMO1P1/SUMO5.
(Microbial infection) Interacts with human herpesvirus 6 IE2.
(Microbial infection) Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation.
(Microbial infection) Interacts with Epstein-barr virus protein LMP1.
Belongs to the ubiquitin-conjugating enzyme family.
研究领域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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