Claudin 1 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from mouse liver, using CLDN1 Antibody.

Fig.

Figure 7 Expression of ZO-1, occludin, and claudin-1 in IEC-6 cells treated by the medium or polysaccharide products (LP, SeLP1, and SeLP2, 10 μg/mL) for 24 h, followed by 40 μmol/L FB1 exposure for 48 h.

Fig.

产品:	Claudin 1 抗体
货号:	DF6919
描述:	Rabbit polyclonal antibody to Claudin 1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量:	23kDa; 23kD(Calculated).
蛋白号:	O95832
RRID:	AB_2838878

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%)

克隆:

Polyclonal

特异性:

Claudin 1 Antibody detects endogenous levels of total Claudin 1.

RRID:

AB_2838878
引用格式: Affinity Biosciences Cat# DF6919, RRID:AB_2838878.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein;

抗原和靶标

免疫原:

Uniprot:

O95832(CLD1_HUMAN) >>Visit HPA database.

基因/基因ID:

CLDN1(9076)

表达:

O95832 CLD1_HUMAN:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

描述:

Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).

序列:

O95832 CLD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Chicken

Dog

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95832 作为底物

O95832

Site	PTM Type	Source
K115	Ubiquitination	Uniprot
T153	Phosphorylation	Uniprot
K189	Ubiquitination	Uniprot
T191	O-Glycosylation	Uniprot
T191	Phosphorylation	Uniprot
S192	O-Glycosylation	Uniprot
S192	Phosphorylation	Uniprot
T195	Phosphorylation	Uniprot
K201	Ubiquitination	Uniprot
S205	O-Glycosylation	Uniprot
S205	Phosphorylation	Uniprot
S206	O-Glycosylation	Uniprot
S206	Phosphorylation	Uniprot
K208	Ubiquitination	Uniprot
Y210	Phosphorylation	Uniprot

研究背景

O95832_CLD1_HUMAN

功能:

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.

组织特异性:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

亚基结构:

Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.

(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.

(Microbial infection) Interacts with dengue virus small envelope protein M.

蛋白家族:

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

文献引用

1). Capsaicin shapes gut microbiota and pre-metastatic niche to facilitate cancer metastasis to liver. Pharmacological Research, 2023 (PubMed: 36608780) [IF=9.3]

2). Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. International Journal of Biological Macromolecules, 2023 (PubMed: 37327932) [IF=8.2]

3). Hypoxia activates SUMO-1-HIF-1α signaling pathway to upregulate pro-inflammatory cytokines and permeability in human tonsil epithelial cells. LIFE SCIENCES, 2021 (PubMed: 33794253) [IF=6.1]

Application: IHC Species: Human Sample: HTEC cells

Fig. 3. SUMO1 silencing contributes to reduction in permeability of HTEC cells under hypoxic condition. (A). Knockdown of SUMO1 inhibited the secretion of IL-6, IL-8 and TNF-α in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!p < 0.01 vs siNC. (B). SUMO1 silencing promoted the TEER value in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!!p < 0.001 vs siNC. (C). SUMO-1 silencing reduced the permeability of HTEC cells treated under hypoxic condition for 24 h. *p < 0.05 vs control, ***p < 0.001 vs siNC; !!!p < 0.001 vs siNC. (D). Western blotting was used to examine the protein levels of SUMO1, HIF-1α, VEGF, Occluding, Claudin-1 and ZO-1 in cells as indicated. (E). Immunohistochemistry staining assay was performed to determine the levels of ZO-1, Occluding and Claudin-1 in cells as indicated above.

Application: WB Species: Human Sample: HTEC cells

4). Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells. Nutrients, 2023 (PubMed: 37960333) [IF=5.9]

Application: WB Species: Rat Sample: IEC-6 cells

5). "Yajieshaba" prevents acute alcoholic liver injury and repairs the intestinal mucosal barrier. Journal of ethnopharmacology, 2024 (PubMed: 37490990) [IF=5.4]

Application: WB Species: Mouse Sample:

Fig. 9. YJSB promoted the expression of alcohol-stimulated ileal mucosal proteins (n = 3). Panel G represents Western blot analysis shown in Fig. 8 , panel H–J represents data shown in Fig. 8 . A indicates the normal group, B indicates the model group, C indicates the Bifendate group, D indicates the YJSB 0.39 g/kg dose group, E indicates the YJSB 1.17 g/kg dose group, F indicates the YJSB 3.51 g/kg dose group, H indicates the expression of claudin1 in the ileum, I indicates the expression of occludin in the ileum, J indicates the expression of ZO-1 in the ileum. YJSB pretreatment upregulated the expression of claudin1, occludin, and ZO-1 in the ileal mucosa; ##p < 0.01 compared to the normal group; **p < 0.01 and *p < 0.05 compared to the model group.

6). Cimifugin improves intestinal barrier dysfunction by upregulating SIRT1 to regulate the NRF2/HO-1 signaling pathway. Naunyn-Schmiedeberg's archives of pharmacology, 2024 (PubMed: 39302422) [IF=3.6]

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Claudin 1 Antibody - #DF6919

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