| 产品: | TTK 抗体 |
| 货号: | DF6969 |
| 描述: | Rabbit polyclonal antibody to TTK |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 97kDa; 97kD(Calculated). |
| 蛋白号: | P33981 |
| RRID: | AB_2838925 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
TTK Antibody detects endogenous levels of total TTK.
RRID:
AB_2838925
引用格式: Affinity Biosciences Cat# DF6969, RRID:AB_2838925.
引用格式: Affinity Biosciences Cat# DF6969, RRID:AB_2838925.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ECSTY kinase; ESK; FLJ38280; hMPS1; Monopolar Spindle 1 Like 1; MPH1; Mps 1; MPS1L1; Phosphotyrosine Picked Threonine Kinase; Phosphotyrosine picked threonine-protein kinase; PYT; RP3-357D13.2; ttk; TTK Protein Kinase; TTK_HUMAN;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
描述:
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
序列:
- P33981 TTK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
75
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33981 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | P33981 (TTK) | Uniprot |
| S15 | Phosphorylation | P33981 (TTK) | Uniprot |
| K19 | Methylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T33 | Phosphorylation | P33981 (TTK) | Uniprot |
| S37 | Phosphorylation | P33981 (TTK) | Uniprot |
| T46 | Phosphorylation | Uniprot | |
| K71 | Sumoylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S80 | Phosphorylation | P33981 (TTK) | Uniprot |
| K86 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S281 | Phosphorylation | P15056 (BRAF) | Uniprot |
| K287 | Sumoylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| T288 | Phosphorylation | O96017 (CHEK2) , P33981 (TTK) | Uniprot |
| S291 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| T360 | Phosphorylation | P33981 (TTK) | Uniprot |
| S362 | Phosphorylation | P33981 (TTK) | Uniprot |
| S363 | Phosphorylation | P33981 (TTK) | Uniprot |
| K367 | Sumoylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K385 | Methylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S436 | Phosphorylation | P15056 (BRAF) , P33981 (TTK) | Uniprot |
| S440 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| Y462 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| R467 | Methylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K472 | Sumoylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| T564 | Phosphorylation | P33981 (TTK) | Uniprot |
| K577 | Ubiquitination | Uniprot | |
| S582 | Phosphorylation | P33981 (TTK) | Uniprot |
| K584 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| T675 | Phosphorylation | P33981 (TTK) | Uniprot |
| T676 | Phosphorylation | P33981 (TTK) | Uniprot |
| S677 | Phosphorylation | P33981 (TTK) | Uniprot |
| K680 | Ubiquitination | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| T686 | Phosphorylation | P33981 (TTK) | Uniprot |
| K696 | Ubiquitination | Uniprot | |
| K708 | Methylation | Uniprot | |
| S742 | Phosphorylation | P33981 (TTK) | Uniprot |
| K762 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| T795 | Phosphorylation | P33981 (TTK) | Uniprot |
| K803 | Ubiquitination | Uniprot | |
| T805 | Phosphorylation | P33981 (TTK) | Uniprot |
| T806 | Phosphorylation | P33981 (TTK) | Uniprot |
| K810 | Ubiquitination | Uniprot | |
| Y811 | Phosphorylation | P33981 (TTK) | Uniprot |
| S821 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) , P33981 (TTK) | Uniprot |
| S824 | Phosphorylation | P33981 (TTK) | Uniprot |
| K827 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| Y836 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| S845 | Phosphorylation | P33981 (TTK) | Uniprot |
| S847 | Phosphorylation | P33981 (TTK) | Uniprot |
| K848 | Ubiquitination | Uniprot | |
| T849 | Phosphorylation | P33981 (TTK) | Uniprot |
| K852 | Acetylation | Uniprot | |
| K853 | Acetylation | Uniprot |
翻译修饰 - P33981 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O60566 (BUB1B) | S435 | Uniprot |
| O60566 (BUB1B) | S543 | Uniprot |
| O60566 (BUB1B) | S670 | Uniprot |
| O60566 (BUB1B) | S1043 | Uniprot |
| O96017 (CHEK2) | T68 | Uniprot |
| P00519 (ABL1) | T735 | Uniprot |
| P04637 (TP53) | T18 | Uniprot |
| P07900 (HSP90AA1) | T115 | Uniprot |
| P33981 (TTK) | T12 | Uniprot |
| P33981 (TTK) | S15 | Uniprot |
| P33981 (TTK) | T33 | Uniprot |
| P33981 (TTK) | S37 | Uniprot |
| P33981 (TTK) | S80 | Uniprot |
| P33981 (TTK) | T288 | Uniprot |
| P33981 (TTK) | T360 | Uniprot |
| P33981 (TTK) | S362 | Uniprot |
| P33981 (TTK) | S363 | Uniprot |
| P33981 (TTK) | S436 | Uniprot |
| P33981 (TTK) | T564 | Uniprot |
| P33981 (TTK) | S582 | Uniprot |
| P33981 (TTK) | T675 | Uniprot |
| P33981 (TTK) | T676 | Uniprot |
| P33981 (TTK) | S677 | Uniprot |
| P33981 (TTK) | T686 | Uniprot |
| P33981 (TTK) | S742 | Uniprot |
| P33981 (TTK) | T795 | Uniprot |
| P33981 (TTK) | T805 | Uniprot |
| P33981 (TTK) | T806 | Uniprot |
| P33981 (TTK) | Y811 | Uniprot |
| P33981 (TTK) | S821 | Uniprot |
| P33981 (TTK) | S824 | Uniprot |
| P33981 (TTK) | S845 | Uniprot |
| P33981 (TTK) | S847 | Uniprot |
| P33981 (TTK) | T849 | Uniprot |
| P38646 (HSPA9) | T62 | Uniprot |
| P38646 (HSPA9) | S65 | Uniprot |
| P54132 (BLM) | S144 | Uniprot |
| P54132 (BLM) | S1290 | Uniprot |
| P54132 (BLM) | S1296 | Uniprot |
| Q53HL2 (CDCA8) | T88 | Uniprot |
| Q53HL2 (CDCA8) | T94 | Uniprot |
| Q53HL2 (CDCA8) | T169 | Uniprot |
| Q53HL2 (CDCA8) | T230 | Uniprot |
| Q53HL2 (CDCA8) | S238 | Uniprot |
| Q96E14 (RMI2) | S112 | Uniprot |
研究背景
功能:
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
组织特异性:
Present in rapidly proliferating cell lines.
亚基结构:
Interacts with TPR; the interactions occurs in a microtubule-independent manner.
蛋白家族:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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