AXIN2 Antibody - #DF6978

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产品: AXIN2 抗体
货号: DF6978
描述: Rabbit polyclonal antibody to AXIN2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 93kDa; 94kD(Calculated).
蛋白号: Q9Y2T1
RRID: AB_2838934

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
AXIN2 Antibody detects endogenous levels of total AXIN2.
RRID:
AB_2838934
引用格式: Affinity Biosciences Cat# DF6978, RRID:AB_2838934.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Axil; Axin like protein; Axin-2; Axin-like protein; Axin2; AXIN2_HUMAN; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582;

抗原和靶标

免疫原:
Uniprot:

Q9Y2T1(AXIN2_HUMAN) >>Visit HPA database.

基因/基因ID:
AXIN2(8313)
表达:
Q9Y2T1 AXIN2_HUMAN:

Expressed in brain and lymphoblast.

描述:
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
序列:
MSSAMLVTCLPDPSSSFREDAPRPPVPGEEGETPPCQPGVGKGQVTKPMSVSSNTRRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSIVSKQLKPATKTYIRDGIKKQQIDSIMFDQAQTEIQSVMEENAYQMFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVVCGYLPTLNEEEEWTCADFKCKLSPTVVGLSSKTLRATASVRSTETVDSGYRSFKRSDPVNPYHIGSGYVFAPATSANDSEISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPATFAAELISRLEKLKLELESRHSLEERLQQIREDEEREGSELTLNSREGAPTQHPLSLLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHSQYHSLLPPGGKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVHCFCPGGSEYYCYSKCKSHSKAPETMPSEQFGGSRGSTLPKRNGKGTEPGLALPAREGGAPGGAGALQLPREEGDRSQDVWQWMLESERQSKPKPHSAQSTKKAYPLESARSSPGERASRHHLWGGNSGHPRTTPRAHLFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQQRDRNHSATVQTGATPFSNPSLAPEDHKEPKKLAGVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWEDETVLPMYEGRILGKVERID

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
92
Chicken
92
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y2T1 作为底物

Site PTM Type Enzyme
Ubiquitination
S70 Phosphorylation
S73 Phosphorylation
K80 Ubiquitination
S146 Phosphorylation
S244 Phosphorylation
T246 Phosphorylation
S311 Phosphorylation P53350 (PLK1)
S348 Phosphorylation
T449 Phosphorylation
S454 Phosphorylation
S461 Phosphorylation
S493 Phosphorylation
K505 Ubiquitination
K528 Ubiquitination
Y549 Phosphorylation
S630 Phosphorylation
K633 Acetylation
S639 Phosphorylation
T640 Phosphorylation
K792 Acetylation
K797 Acetylation
K806 Acetylation
K838 Ubiquitination

研究背景

功能:

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

翻译修饰:

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in brain and lymphoblast.

亚基结构:

Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin (By similarity). Interacts with SMAD7 and RNF111. Interacts with ANKRD6.

蛋白家族:

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Hederagenin ameliorates cisplatin-induced acute kidney injury via inhibiting long non-coding RNA A330074k22Rik/Axin2/β-catenin signalling pathway. International Immunopharmacology, 2022 (PubMed: 36155281) [IF=5.6]

Application: WB    Species: Mice    Sample: pTEC cells

Fig. 5. Transcriptome sequencing analysis of differential mRNAs after downregulation of A33 in LPS-stimulated pTEC cells (A) Violin plot of gene expression; (B) Based on the results of differential analysis, we screened genes with FDR < 0.05 and |log2FC|>1 as significantly differential genes, and the statistical histogram of differential genes. (C) The different genes volcano plot of LPS + siR-A33 vs LPS; (D) KEGG enrichment: the top 20 pathways with the smallest Q value. Inflammation-related pathways are in the red box, and pathways in which Wnt signaling first appears are in the blue box; (E) Heatmap of gene expression associated with wnt/β-catenin signaling; (F) Western blot analysis of Axin2 and β-catenin protein levels after down-regulation of A33. ***P < 0.001 vs LPS group.

Application: IHC    Species: Mice    Sample:

Fig. 6. HDG strongly inhibited the expression of Axin2 and β-catenin in vivo and in vitro, and A33 was the upstream regulatory LncRNA of Axin2 and β-catenin. (A) Immunohistochemistry results for Axin2 and β-catenin in each group; (B,C) Real-time PCR and WB results confirmed that mRNA and protein levels of Axin and β-catenin decreased significantly in HDG treatment group; (D) Immunohistochemistry results of Axin2 and β-catenin after knockdown of A33 in vivo; (E,F) Real-time PCR and WB results confirmed that HDG had a significantly inhibitory effect on Axin2 and β-catenin in LPS-induced pTEC; (G,H) Real-time PCR and WB confirmed that the expression of Axin2/β-catenin was synergistic with the expression of A33 after knockdown and over-expression in vitro. *P < 0.05, **P < 0.01, ***P < 0.001 vs Cis/LPS group; #P < 0.05, ###P < 0.001 vs Ctrl group.
2). Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells. Bioengineered, 2021 (PubMed: 34823423) [IF=4.9]
3). Gigantol Attenuates the Metastasis of Human Bladder Cancer Cells, Possibly Through Wnt/EMT Signaling. OncoTargets and Therapy, 2020 (PubMed: 33177841) [IF=4.0]

Application: WB    Species: Human    Sample: Bladder cancer cell

Figure 3 Cell invasion assays and expression analysis of Wnt/EMT related genes in cells treated with gigantol. (A) Cell invasion was measured by transwell assay in bladder cancer cells treated with increasing concentrations of gigantol (magnification ×100). (B) qRT-PCR analysis of the Wnt target genes and EMT markers in bladder cancer cells treated with gigantol. (C) Western blot analysis of Wnt/EMT markers in bladder cancer cells treated with indicated concentrations of gigantol. Data in (B) were shown as means ± SD (n = 3), the statistically significant differences were considered at *p<0.05, **p<0.01, ***p<0.001.
4). Wnt and Smad signaling pathways synergistically regulated the osteogenic differentiation of fibroblasts in ankylosing spondylitis. TISSUE & CELL, 2022 (PubMed: 35753224) [IF=2.6]
5). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.6]

Application: WB    Species: Human    Sample: AS fibroblasts

Fig. 3 Proteins expression of Wnt and Smad signaling pathways in AS fibroblasts after different treatment. A Protein expressions of Wnt, β-catenin, GSK-3β, Axin, Smad3 and p-Smad3 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of Wnt protein expression. C Densitometry analysis of β-catenin protein expression. D Densitometry analysis of GSK-3β protein expression. E Densitometry analysis of Axin protein expression. F Densitometry analysis of p-Smad/Smad protein expression. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group
6). Golden pompano genome resource enables discovery of valuable gene determining growth traits. bioRxiv, 2021

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