产品: IL8 抗体
货号: DF6998
来源: Rabbit
应用: WB, IHC, ELISA(peptide)
反应: Human
分子量: 11kD; 11kD(Calculated).
蛋白号: P10145
RRID: AB_2838954

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
IL8 Antibody detects endogenous levels of total IL8.
RRID:
AB_2838954
引用格式: Affinity Biosciences Cat# DF6998, RRID:AB_2838954.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

(Ala-IL-8)77; (Ser-IL-8)72; 9E3; Beta thromboglobulin like protein; C-X-C motif chemokine 8; CEF-4; chemokine, CXC motif, ligand 8; CXCL8; Emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; Granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(9-77); IL8; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; IL8_HUMAN; Inteleukin 8; LECT; LUCT; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP-1; NAP1; Neutrophil activating peptide 1; Neutrophil activating protein 1; Neutrophil-activating factor; Neutrophil-activating protein 1; Protein 3 10C; Protein 3-10C; SCYB8; Small inducible cytokine subfamily B member 8; T cell chemotactic factor; T-cell chemotactic factor; TSG1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
蛋白描述:
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
蛋白序列:
MTSKLAVALLAAFLISAALCEGAVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS

研究背景

功能:

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

翻译修饰:

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the intercrine alpha (chemokine CxC) family.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). Zhao M et al. HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury. Cell Mol Biol Lett 2021 Sep 3;26(1):40. (PubMed: 34479471) [IF=8.702]

Application: WB    Species: Human    Sample: HUVECs

Fig. 3 Effects of inhibition of HIF-1α on expression of inflammation after hyperglycemic and hypoxia. a Cells were treated with glucose (25 mM) or with combined exposure to high glucose and hypoxia for 6, 12, 24, and 48 h. Exposure to glucose alone or combined stimulation with hypoxia significantly increased gene expression of HIF-1α. b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8, ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression. Importantly, the downregulation of HIF-1α decreased the secretion of IL-6, IL-8, ICAM-1 and MCP-1 based on ELISA and qRT-PCR assays (e–f). n = 3; *p < 0.05 and **p < 0.01 vs. DMSO. DMSO-treated cells, DMSO; HIF-1α inhibitors KC7F2 (10 µM)-treated cells, KC7F2 (10 µM); si-HIF-1α, small interfering RNA HIF-1α; HG, high glucose; HG + Hypoxia, combined stimulus with high glucose and hypoxia; NG, control

Application: WB    Species: human    Sample: HUVECs

Fig. 3 | Efects of inhibition of HIF-1α on expression of infammation after hyperglycemic and hypoxia.b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8,ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression.

2). Qin X et al. An Extracellular Matrix-Mimicking Hydrogel for Full Thickness Wound Healing in Diabetic Mice. Macromol Biosci 2018 Jul;18(7):e1800047 (PubMed: 29737012) [IF=5.859]

Application: IHC    Species: rat    Sample: inflammatory cell

Figure 6. |Immunohistochemical staining of a) IL-8 and b) MCP-1 in the wound bed on day 3, 7, and 14. Scale bars represent 100 µm. c) Semiquantitative analysis of IL-8 and MCP-1, double-blind analysis by using a four-point scale (0, no positive cells; 1, low number of positive cells; 2, moderate number of positive cells; and 3, high number of positive cells). The level of IL-8 in the HA-GEL group was significantly lower than that in the other two groups at day 7 (p < 0.05). On day 3, both alginate and HA-GEL groups could significantly reduce the expression of MCP-1. On day 7, the level of MCP-1 in the HA-GEL group remained significantly lower than that in the alginate group and control.

3). Lin L et al. BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway. Int Immunopharmacol 2020 May 30;85:106625. (PubMed: 32485356) [IF=5.714]

Application: IHC    Species: mouse    Sample: tumor

Fig.7. The inhibitory effect of BML-111 on the tumor growth of 4T1-MIND model mice in vivo (A) Representative images of the tumor tissue dissected from 4T1-MIND model mice. *P = 0.0271 BML-111 group vs. control group. (F) The expression of IL-8 in tumor cells and stroma of BML-111 group was compared with that in control group. Scale bar, 200 μm. ****P < 0.0001.

4). Li J et al. Transcriptional Profiling Reveals the Regulatory Role of CXCL8 in Promoting Colorectal Cancer. Front Genet 2020 Jan 21;10:1360 (PubMed: 32038715) [IF=4.599]

Application: IHC    Species: human    Sample: tumor

FIGURE 3 | Expression levels of CXCL8 in the clinical cohort. (A) The levels of CXCL8 mRNA in the normal (N = 81), colorectal cancer (CRC)-early stage (N = 44)and CRC-advanced stage (N = 37) groups. (B) The levels of CXCL8 protein in normal (N = 87), CRC-early stage (N = 48), and CRC-advanced stage (N = 39)groups. (C) Immunohistochemical staining of normal, CRC-early stage and CRC-advanced stage groups.

5). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736) [IF=4.136]

Application: WB    Species: mouse    Sample: TAMs

Figure 2. |PRL‑3‑induced activation of IL‑6 and IL‑8 by initiating JNK and ERK pathways in TAMs. (A) After co‑culture with colorectal cancer cells, IL‑6, IL‑8, p‑JNK, and p‑ERK levels in TAMs were examined using western blot assays.

6). Ding W et al. Alamandine, a new member of the renin-angiotensin system (RAS), attenuates collagen-induced arthritis in mice via inhibiting cytokine secretion in synovial fibroblasts. Peptides 2022 Aug;154:170816. (PubMed: 35609788) [IF=3.867]

7). Wang P et al. Screening of immunosuppressive factors for biomarkers of breast cancer malignancy phenotypes and subtype-specific targeted therapy. PeerJ 2019 Jun 27;7:e7197 (PubMed: 31293831) [IF=3.061]

Application: WB    Species: human    Sample: T549 and MDA-MB-231 cell; MCF7 and T47D cell

Figure 8| qRT-PCR and western blot results. (A, B) qRT-PCR results showed that CD274 and IL8 were upregulated in the basal-like breast cancer cell lines BT549 and MDA-MB-231 (p < 0.0001). Similar to the qRT-PCR results, the western blot analysis (C–E) indicated that CD274 and IL8 protein were increased in the BT549 and MDA-MB-231 cell lines compared to the MCF7 and T47D cell lines. P < 0.001, p < 0.01, and p < 0.05

8). Huang SP et al. Evaluation of the Mechanism of Jiedu Huazhuo Quyu Formula in Treating Wilson’s Disease-Associated Liver Fibrosis by Network Pharmacology Analysis and Molecular Dynamics Simulation. Evid Based Complement Alternat Med 2022 Jun 6;2022:9363131. (PubMed: 35707473) [IF=2.650]

Application: WB    Species: Human    Sample: LX-2 cells

Figure 7 Effect of JHQ on core target proteins abundant in LX-2 cells after CuSO4 treatment. ∗∗∗P < 0.001 and ∗∗P < 0.01 compared with the model group.

9). Zhao B et al. Prostatic fluid exosome-mediated microRNA-155 promotes the pathogenesis of type IIIA chronic prostatitis. Transl Androl Urol 2021 May;10(5):1976-1987. (PubMed: 34159078) [IF=2.479]

Application: IF/ICC    Species: Rat    Sample: prostate tissue

Figure 4 Prostatic fluid exosomes promoted inflammation in prostate tissue. (A) Representative HE-staining images of prostate tissue in SD rats injected with normal saline, NC exosomes, prostatic fluid exosomes of CP/CPPS-A patients, and ultrasound-treated prostatic fluid exosomes of CP/CPPS-A patients. (B) Results of prostate tissue in different groups measured by MPO activity assay. (C–E) Expression of IL-8 (TRITC secondary antibodies), TNF-α (FITC secondary antibodies), and iNOS (TRITC secondary antibodies) in different groups of prostate tissue observed under a fluorescence microscope, scale bar =100 µm. *, P<0.05. NS, normal saline; NC, normal control; CP/CPPS-A, type IIIA chronic prostatitis/chronic pelvic pain syndrome; CP/CPPS-A + US, ultrasonic treatment in the CP/CPPS-A; MPO, myeloperoxidase.

10). Zhuoqi Liu et al. Screening of Immunosuppressive Factors for Identifying Breast Cancer Malignant Phenotypes Using mRNA Microarray Datasets. Preprints 2016; 2016090124

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