FES Antibody - #DF7031

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产品: FES 抗体
货号: DF7031
描述: Rabbit polyclonal antibody to FES
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep
分子量: 93kDa; 93kD(Calculated).
蛋白号: P07332
RRID: AB_2838987

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(86%), Horse(86%), Sheep(86%)
克隆:
Polyclonal
特异性:
FES Antibody detects endogenous levels of total FES.
RRID:
AB_2838987
引用格式: Affinity Biosciences Cat# DF7031, RRID:AB_2838987.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

c-fes/fps protein; cFES; Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog; feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami; Feline sarcoma oncogene; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; fes; FES proto oncogene, tyrosine kinase; FES_HUMAN; FPS; Oncogene FES; Oncogene FES feline sarcoma virus; P93c fes; Proto oncogene tyrosine protein kinase Fes/Fps; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; Tyrosine-protein kinase Fes/Fps;

抗原和靶标

免疫原:
Uniprot:

P07332(FES_HUMAN) >>Visit HPA database.

基因/基因ID:
FES(2242)
表达:
P07332 FES_HUMAN:

Widely expressed. Detected in adult colon epithelium (at protein level) (PubMed:16455651, PubMed:19051325). Expressed in melanocytes (at protein level) (PubMed:28463229).

描述:
This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.
序列:
MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHSTSSSEQEREGGRTPTLEILKSHISGIFRPKFSLPPPLQLIPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLWDGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
86
Bovine
86
Sheep
86
Zebrafish
71
Chicken
71
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07332 作为底物

Site PTM Type Enzyme
S64 Phosphorylation
S67 Phosphorylation
S100 Phosphorylation
S149 Phosphorylation
Y156 Phosphorylation
Y261 Phosphorylation
S373 Phosphorylation
S408 Phosphorylation
T409 Phosphorylation
S410 Phosphorylation
S411 Phosphorylation
S412 Phosphorylation
T421 Phosphorylation
T423 Phosphorylation
S432 Phosphorylation
Y461 Phosphorylation
S477 Phosphorylation
T595 Phosphorylation
Y614 Phosphorylation
K698 Ubiquitination
Y713 Phosphorylation P07332 (FES)
S716 Phosphorylation
K725 Ubiquitination
Y734 Phosphorylation
K775 Ubiquitination
Y811 Phosphorylation P07332 (FES)

翻译修饰 - P07332 作为激酶

Substrate Site Source
P07332 (FES) Y713 Uniprot
P07332 (FES) Y811 Uniprot
P11274 (BCR) Y177 Uniprot
P11274-1 (BCR) Y246 Uniprot
P11274 (BCR) Y276 Uniprot
P11274 (BCR) Y279 Uniprot
P11274 (BCR) Y283 Uniprot
P46459 (NSF) Y83 Uniprot

研究背景

功能:

Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down-stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.

翻译修饰:

Autophosphorylated on Tyr-713. Phosphorylated by LYN in response to FCER1 activation. Phosphorylated by HCK.

细胞定位:

Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Golgi apparatus. Cell junction>Focal adhesion.
Note: Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Detected in adult colon epithelium (at protein level). Expressed in melanocytes (at protein level).

亚基结构:

Homooligomer. Interacts with BCR. Interacts (when activated, via coiled coil domain) with TRIM28. Interacts (via SH2 domain) with phosphorylated EZR, MS4A2/FCER1B and HCLS1/HS1. Interacts with phosphorylated KIT. Interacts with FLT3. Interacts (via F-BAR domain) with soluble tubulin. Interacts (via SH2 domain) with microtubules.

蛋白家族:

The coiled coil domains are important for regulating the kinase activity. They mediate homooligomerization and probably also interaction with other proteins.

The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes.

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

研究领域

· Organismal Systems > Development > Axon guidance.   (View pathway)

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